Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,208,517	C→T	P129L (CCT→CTT)  *201* (TAG→TAA)	tfaP → tfaE ←	e14 prophage, uncharacterized protein e14 prophage, putative tail fiber assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,208,517	0	C	T	100.0%	115.0 / NA	38	P129L (CCT→CTT)  *201* (TAG→TAA)	tfaP tfaE	e14 prophage, uncharacterized protein e14 prophage, putative tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/17);  total (21/17)

TAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAAT  >  NC_000913/1208383‑1208652                                                                                                                                       |                                                                                                                                        tAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACg                                                                                                                                     >  2:734207/1‑139 (MQ=255)           tGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAg                                                                                                                           >  2:259/1‑139 (MQ=255)                gggTAAGGCTGCATTGGTAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTGTCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGttagt                                                                                                                      <  2:491802/139‑4 (MQ=255)                     aGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGa                                                                                                                >  1:391536/1‑139 (MQ=255)                     aGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGa                                                                                                                >  1:680549/1‑139 (MQ=255)                                 gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                    <  1:259/139‑1 (MQ=255)                                 gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                    <  2:546452/139‑1 (MQ=255)                                 gAAGACTTAAAGAAGATGAGCTAAAAAATTATAATTTGTGGCTGGATTATCTGGAAGCACTGAAACTGGTTGATACATCCAGTGCGCCAAATATTGAATGGCTTACCCCTCCGGCAGTT‑AGGCCAGATGACATCCgggg                                                                                                    <  1:734207/139‑3 (MQ=18)                                        tAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCt                                                                                             >  2:234219/1‑139 (MQ=255)                                          aagaagATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTg                                                                                               >  2:631911/1‑135 (MQ=255)                                          aagaagATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTg                                                                                               <  1:631911/135‑1 (MQ=255)                                               gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGTAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                    >  1:163176/1‑125 (MQ=255)                                               gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGTAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                    <  2:163176/125‑1 (MQ=255)                                               gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCt                                                                                      >  1:482244/1‑139 (MQ=255)                                                     cTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGcc                                                                                >  1:767994/1‑139 (MQ=255)                                                              tataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGcgcg                                                                                                  <  2:379595/112‑1 (MQ=255)                                                              tataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGcgcg                                                                                                  >  1:379595/1‑112 (MQ=255)                                                              tataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACcgcg                                                                       >  2:295867/1‑139 (MQ=255)                                                               ataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                                      <  1:234219/139‑1 (MQ=255)                                                               ataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                                      <  2:242359/139‑1 (MQ=255)                                                                                 aTCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAg                                                    >  2:256002/1‑139 (MQ=255)                                                                                       aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATc                                                            <  1:17979/125‑1 (MQ=255)                                                                                       aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATc                                                            >  2:17979/1‑125 (MQ=255)                                                                                             tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTgg                                        <  2:158182/139‑1 (MQ=255)                                                                                             tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTgg                                        <  1:267948/139‑1 (MQ=255)                                                                                                aaCTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGc                                                        >  1:548835/1‑120 (MQ=255)                                                                                                aaCTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGc                                                        <  2:548835/120‑1 (MQ=255)                                                                                                aaCTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGgttg                                     >  2:152424/1‑139 (MQ=255)                                                                                                    ggTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGc                                                        <  1:59046/116‑1 (MQ=255)                                                                                                    ggTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGc                                                        >  2:59046/1‑116 (MQ=255)                                                                                                               ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCa                      >  1:42937/1‑139 (MQ=255)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGttaggc                                                                                                                     <  1:626627/41‑5 (MQ=14)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGttaggc                                                                                                                     >  2:626627/1‑37 (MQ=14)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt                  >  2:274280/1‑139 (MQ=255)                                                                                                                       ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                    <  1:7808/53‑1 (MQ=14)                                                                                                                       ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                    >  2:7808/1‑53 (MQ=14)                                                                                                                       ccAGATATTGAATGACTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGt              >  2:395716/1‑139 (MQ=255)                                                                                                                                   tGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTTACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAAt  <  2:482244/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAAT  >  NC_000913/1208383‑1208652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑