Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,212,703	C→T	*135* (TAG→TAA)	ycgX ←	DUF1398 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,212,703	0	C	T	100.0%	142.3 / NA	44	*135* (TAG→TAA)	ycgX	DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/20);  total (24/20)

GTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTG  >  NC_000913/1212576‑1212830                                                                                                                                |                                                                                                                                gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATGATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTCCTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATgg                                                                                                                      >  2:463044/1‑139 (MQ=255) gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATgg                                                                                                                      >  1:272434/1‑139 (MQ=255) gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATgg                                                                                                                      >  2:69355/1‑139 (MQ=255)  tGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGt                                                                                                                     <  2:745708/139‑1 (MQ=255)                  aCAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACAt                                                                                                     <  1:396869/139‑1 (MQ=255)                  aCAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACAt                                                                                                     >  2:259755/1‑139 (MQ=255)                     aTAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCtt                                                                                                  <  2:450248/139‑1 (MQ=255)                       aaaaaGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCa                                                                                                <  2:445866/139‑1 (MQ=255)                       aaaaaGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCa                                                                                                <  1:347516/139‑1 (MQ=255)                          aaGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAata                                                                                             <  2:247167/139‑1 (MQ=255)                              cAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAATTATCAAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAAtatata                                                                                         >  1:666197/1‑139 (MQ=255)                              cAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCa                                                                                                <  2:792010/132‑1 (MQ=255)                              cAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCa                                                                                                >  1:792010/1‑132 (MQ=255)                                 tATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATaaaa                                                                                      >  2:383861/1‑139 (MQ=255)                                     aaTCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGt                                                                                  <  2:788938/139‑1 (MQ=255)                                     aaTCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGt                                                                                  <  2:749402/139‑1 (MQ=255)                                      atcatcCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGta                                                                                 <  1:69355/139‑1 (MQ=255)                                       tcatcCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGtat                                                                                <  2:798744/139‑1 (MQ=255)                                           ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTAc                                                                                                       <  2:645014/112‑1 (MQ=255)                                           ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTAc                                                                                                       >  1:645014/1‑112 (MQ=255)                                           ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAACGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATc                                                                            >  2:336591/1‑139 (MQ=255)                                                     aGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCATTGGCgtag                                                                  >  1:39381/1‑139 (MQ=255)                                                         tctGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAAc                                                              >  2:483150/1‑139 (MQ=255)                                                            gCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGc                                                           >  1:248592/1‑139 (MQ=255)                                                                    aTTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTa                                                   <  1:95091/139‑1 (MQ=255)                                                                           ttATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTaa                                            <  2:248592/139‑1 (MQ=255)                                                                            tATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAAc                                           <  2:519906/139‑1 (MQ=255)                                                                                          aCTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAAc                                           <  2:8833/125‑1 (MQ=255)                                                                                          aCTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAAc                                           >  1:8833/1‑125 (MQ=255)                                                                                              aaTTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGaa                         >  2:450979/1‑139 (MQ=255)                                                                                                ttATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGaaaa                       >  1:58222/1‑139 (MQ=255)                                                                                                   tGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTa                                                   >  1:795134/1‑108 (MQ=255)                                                                                                   tGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTa                                                   <  2:795134/108‑1 (MQ=255)                                                                                                     aaGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGccc                  >  2:125677/1‑139 (MQ=255)                                                                                                          tttCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGcc             >  1:727845/1‑139 (MQ=255)                                                                                                           ttCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCt            >  1:746945/1‑139 (MQ=255)                                                                                                                  gTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAgtg     <  2:666197/139‑1 (MQ=255)                                                                                                                  gTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTACCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAgtg     >  2:270679/1‑139 (MQ=255)                                                                                                                     tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCa                                                                                                >  1:86505/1‑45 (MQ=255)                                                                                                                     tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCa                                                                                                <  2:86505/45‑1 (MQ=255)                                                                                                                     tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATaaaa                                                                                      >  2:378354/1‑55 (MQ=255)                                                                                                                     tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATaaaa                                                                                      <  1:378354/55‑1 (MQ=255)                                                                                                                     tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTg  >  2:635953/1‑139 (MQ=255)                                                                                                                     tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTg  >  1:336442/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                GTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTG  >  NC_000913/1212576‑1212830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑