Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,264,970	G→A	*419* (TAG→TAA)	hemA →	glutamyl tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,264,970	0	G	A	100.0%	95.2 / NA	30	*419* (TAG→TAA)	hemA	glutamyl tRNA reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/17);  total (13/17)

CAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAA  >  NC_000913/1264845‑1265092                                                                                                                              |                                                                                                                           cAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCAttt                                                                                                               <  2:365798/139‑1 (MQ=255)         ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCtttttt                                                                                                       >  1:511077/1‑139 (MQ=255)           cATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa                                                                                                     <  2:511077/139‑1 (MQ=255)           cATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa                                                                                                     <  1:550450/139‑1 (MQ=255)               gAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAgg                                                                                                 <  1:46303/139‑1 (MQ=255)                aaaCTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAggg                                                                                                >  1:211786/1‑139 (MQ=255)                 aaCTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGt                                                                                               <  1:302891/139‑1 (MQ=255)                         aaCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggagg                                                                                       >  2:720879‑M1/1‑132 (MQ=255)                                      aTGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatat                                                                          <  2:211786‑M1/139‑21 (MQ=255)                                       tGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTAAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa                                                                                                     >  1:441922/1‑111 (MQ=255)                                       tGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTAAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa                                                                                                     <  2:441922/111‑1 (MQ=255)                                        gcgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatc                                                                         <  2:691183‑M1/138‑22 (MQ=255)                                        gcgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatc                                                                         >  1:691183‑M1/1‑117 (MQ=255)                                         cgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaa                                                                       <  2:647980‑M1/139‑24 (MQ=255)                                          gcCAACGAAATCACTTCAAGAGGACGTCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCTACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTGTTTACAGGGTGgaggaggaataatggaatatcaac                                                                      <  1:707999‑M1/139‑25 (MQ=255)                                            cAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTa                                                                                                                       <  1:187725/88‑1 (MQ=255)                                            cAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTa                                                                                                                       >  2:187725/1‑88 (MQ=255)                                             aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacact                                                                   >  2:278751‑M1/1‑112 (MQ=255)                                              aCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTg                                                                                              >  1:204011/1‑111 (MQ=255)                                              aCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTg                                                                                              <  2:204011/111‑1 (MQ=255)                                              aCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaaca                                                                     >  2:265771‑M1/1‑111 (MQ=255)                                              aCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaaca                                                                     <  1:265771‑M1/136‑26 (MQ=255)                                                                  cgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagcc                                              <  1:720879‑M1/139‑49 (MQ=255)                                                                               gATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAggg                                                                                                <  1:571729/76‑1 (MQ=255)                                                                               gATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAggg                                                                                                >  2:571729/1‑76 (MQ=255)                                                                                           cTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcg                     <  1:422562‑M1/139‑74 (MQ=255)                                                                                                     tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgct           >  2:101627‑M1/1‑56 (MQ=255)                                                                                                     tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg                                                                               >  2:296241‑M1/1‑56 (MQ=255)                                                                                                     tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg                                                                               <  1:296241‑M1/71‑16 (MQ=255)                                                                                                              gCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctg  >  2:60720‑M1/1‑47 (MQ=255)                                                                                                                              |                                                                                                                           CAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAA  >  NC_000913/1264845‑1265092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑