Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,389,242	C→T	Q105* (CAA→TAA)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,389,242	0	C	T	100.0%	108.2 / NA	35	Q105* (CAA→TAA)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/23);  total (12/23)

CAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACT  >  NC_000913/1389110‑1389372                                                                                                                                     |                                                                                                                                   cAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCgc                                                                                                                              >  1:465998/1‑139 (MQ=255)              tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCTCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                                 <  2:24948/139‑1 (MQ=255)                gtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCg                                                                                                               >  1:274071/1‑139 (MQ=255)                 tGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCgg                                                                                                              >  2:226449/1‑139 (MQ=255)                        aCTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                       <  2:709359/139‑1 (MQ=255)                             agaAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTcc                                                                                                  <  1:226449/139‑1 (MQ=255)                                 aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                              <  2:633474/139‑1 (MQ=255)                                 aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                              <  2:322023/139‑1 (MQ=255)                                      tGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                         <  1:476467/139‑1 (MQ=255)                                       gACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATGGTGGGATCTGGCCGCGCGAAGACAGCAGTAGTCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGtt                                                                                        >  1:495219/1‑139 (MQ=255)                                         cacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGGAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                      <  2:246288/139‑1 (MQ=255)                                             ggaggaGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACt                                                                                  <  1:145939/139‑1 (MQ=255)                                             ggaggaGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACt                                                                                  >  2:145939/1‑139 (MQ=255)                                               aggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGc                                                                                <  2:388255/139‑1 (MQ=255)                                                      gCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACg                                                                         >  2:418661/1‑139 (MQ=255)                                                            aaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                                 <  1:701635/93‑1 (MQ=255)                                                            aaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGAt                                                                                                                 >  2:701635/1‑93 (MQ=255)                                                                 tcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                              <  1:122837/139‑1 (MQ=255)                                                                    cTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                           <  2:501037/139‑1 (MQ=255)                                                                            acaacaCGTAATGCGCTGGATTGTGGATTGTGTGATCTGTACGCGCGAAGACAGCAGTAATCCCGGGCAGATTTGAGCGGCATAACTCTGCTCGAGACCGGTAACACTGAACAGACGGTTGTCATCGGTACGCCTGagc                                                   <  1:272146/135‑3 (MQ=255)                                                                             cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCa                                                  >  1:215448/1‑139 (MQ=255)                                                                                       tGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAAt                                        <  1:390855/139‑1 (MQ=255)                                                                                         cgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                      <  2:215448/139‑1 (MQ=255)                                                                                         cgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                      <  2:431450/139‑1 (MQ=255)                                                                                                 tgtgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCaa                              <  1:617382/139‑1 (MQ=255)                                                                                                   tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGc                                            <  1:414922/123‑1 (MQ=255)                                                                                                   tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGc                                            >  2:414922/1‑123 (MQ=255)                                                                                                        tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                 <  2:28582/113‑1 (MQ=255)                                                                                                        tgtgGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                 >  1:28582/1‑113 (MQ=255)                                                                                                                  ggCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCaggcaggc             >  2:136412/1‑139 (MQ=255)                                                                                                                   gCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGgcg            <  1:136412/139‑1 (MQ=255)                                                                                                                   gCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGgcg            <  2:465998/139‑1 (MQ=255)                                                                                                                       cgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                           <  1:731864/88‑1 (MQ=255)                                                                                                                       cgcgAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                           >  2:731864/1‑88 (MQ=255)                                                                                                                             gACAGCAGTAATCGCTGGCTGATTTGGTCGGCATAACGCTTCCGGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGAGCATCAACACTCTGGCAGGCAGGCGCGAAATTACt  <  1:305601/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   CAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACT  >  NC_000913/1389110‑1389372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑