Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,344	G→A	A38V (GCT→GTT)	ydaG ←	Rac prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,344	0	G	A	100.0%	88.7 / NA	28	A38V (GCT→GTT)	ydaG	Rac prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/13);  total (15/13)

TTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCC  >  NC_000913/1419214‑1419469                                                                                                                                   |                                                                                                                              ttGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTct                                                                                                                       <  2:149447/139‑1 (MQ=255)              aCGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGAt                                                                                                          >  2:738845/1‑139 (MQ=255)               cGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATc                                                                                                         <  1:77934/139‑1 (MQ=255)                   aTGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAg                                                                                                     >  2:785655/1‑139 (MQ=255)                     ggCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTc                                                                                                   >  2:268976/1‑139 (MQ=255)                          tGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgc                                                                                              >  1:549992/1‑139 (MQ=255)                            aaCGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgctg                                                                                            >  2:770566/1‑139 (MQ=255)                               gTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGt                                                                                         <  1:738845/139‑1 (MQ=255)                                         ggTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGca                                                                               <  2:245841/139‑1 (MQ=255)                                          gTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGcac                                                                              >  2:494781/1‑139 (MQ=255)                                                     cgccgcACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGt                                                                   <  1:785655/139‑1 (MQ=255)                                                                       cGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                 >  2:195064/1‑139 (MQ=255)                                                                       cGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                 >  2:658214/1‑139 (MQ=255)                                                                       cGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                 >  2:620820/1‑139 (MQ=255)                                                                                    gTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTg                                    >  1:567750/1‑139 (MQ=255)                                                                                    gTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTg                                    >  1:402896/1‑139 (MQ=255)                                                                                              tGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCg                          <  1:49004/139‑1 (MQ=255)                                                                                               gCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGt                         <  1:658214/139‑1 (MQ=255)                                                                                                  ttttGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAAt                      <  2:452452/139‑1 (MQ=255)                                                                                                      gCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCATTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCAc                  <  2:425034/139‑1 (MQ=255)                                                                                                      gCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCAc                  <  1:494781/139‑1 (MQ=255)                                                                                                              cacaTCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTAt          <  1:146135/139‑1 (MQ=255)                                                                                                                      gttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGaa                                  >  1:69637/1‑107 (MQ=255)                                                                                                                      gttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGaa                                  <  2:296299/107‑1 (MQ=255)                                                                                                                      gttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGaa                                  <  2:69637/107‑1 (MQ=255)                                                                                                                      gttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGaa                                  >  1:296299/1‑107 (MQ=255)                                                                                                                      gttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTcccc  >  2:482034/1‑139 (MQ=255)                                                                                                                      gttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTcccc  >  1:51793/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                              TTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCC  >  NC_000913/1419214‑1419469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑