| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,450,392 | C→T | A320T (GCA→ACA) | tynA ← | tyramine oxidase, copper‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,450,392 | 0 | C | T | 94.4% | 49.1 / ‑6.1 | 18 | A320T (GCA→ACA) | tynA | tyramine oxidase, copper‑requiring |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/8); minor base A (1/0); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCC > NC_000913/1450277‑1450490 | cAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCg > 2:792581/1‑139 (MQ=255) gCCAGTGAATCATATCGCCAGTAATAGTGTCATCTTTACCTCCAGGCGCAATGCCTTGGCTAGGCTCCACTGCCGGAGCAACACGGTCAGGGCCCTCAAAAGGGCGAGTGGTCGTTGGCACCGGAACTACCGGGCctta > 1:269120/1‑138 (MQ=255) tatCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAAc > 2:721311/1‑139 (MQ=255) tAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt > 1:417531/1‑139 (MQ=255) tgtAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTc > 2:728505/1‑139 (MQ=255) cTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGccacc < 2:526394/139‑1 (MQ=255) gCATAGGCTTAACTGCCGGAGTAACGCGTTCACGGCCATCAGATGGGCGTGTGGTCATTGGCAACGGAACTACCGGACCTTCTTCTAGCTTAACGATTTTTTTCTGTTGTAAATCAACGACCGCCACCAGGTTTTCGAt < 1:724734/139‑1 (MQ=255) gCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGAt < 2:799919/139‑1 (MQ=255) gCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAtg < 2:417531/139‑1 (MQ=255) gCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAtg > 1:390067/1‑139 (MQ=255) aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCgg > 2:117721/1‑56 (MQ=255) aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCgg < 1:117721/56‑1 (MQ=255) aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGacc > 2:422046/1‑139 (MQ=255) cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCAc < 2:336154/48‑1 (MQ=255) cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCAc > 1:336154/1‑48 (MQ=255) cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt > 1:734442/1‑128 (MQ=255) cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt < 2:734442/128‑1 (MQ=255) gggCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCtt < 2:524654/47‑1 (MQ=255) gggCGTGAGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCtt > 1:524654/1‑47 (MQ=255) | CAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCC > NC_000913/1450277‑1450490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |