Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,459,052	G→A	*263* (TAG→TAA)	paaG →	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,459,052	0	G	A	100.0%	80.7 / NA	27	*263* (TAG→TAA)	paaG	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/13);  total (14/13)

CGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCG  >  NC_000913/1458917‑1459181                                                                                                                                        |                                                                                                                                  cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACat                                                                                                                                >  2:355951/1‑139 (MQ=255) cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACat                                                                                                                                >  1:52486/1‑139 (MQ=255)  gcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACatg                                                                                                                               >  1:741429/1‑139 (MQ=255)            aTACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtg                                                                                                                     >  2:272725/1‑139 (MQ=255)                  tCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCaaa                                                                                                               <  1:434390/139‑1 (MQ=255)                         gCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTACACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTgg                                                                                                         >  1:582050/1‑138 (MQ=255)                         gCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTgg                                                                                                         <  2:582050/138‑1 (MQ=255)                                gATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGAtt                                                                                                 >  2:238288/1‑139 (MQ=255)                                    tGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGa                                                                                             <  1:238288/139‑1 (MQ=255)                                     ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                            <  2:771988/139‑1 (MQ=255)                                     ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                            <  1:686177/139‑1 (MQ=255)                                       tatgTGACAATCAGCGACTTGCCGGATGGAGAGCATATCATCGTGAAGGTGCCAGTGCGTTCCTGGCTAAACGCTCACTGCAGTTCTCGGGGAAATAACATGATGATAAATGTGCAAACTGTAGCAGTGATTGGGAGCg                                                                                          >  1:795037/4‑139 (MQ=255)                                        aCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                         >  2:241991/1‑139 (MQ=255)                                           tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAc                                                                                      >  1:771214/1‑139 (MQ=255)                                               tATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATg                                                                                  <  2:233733/139‑1 (MQ=255)                                                       aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                          <  2:52486/139‑1 (MQ=255)                                                           gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                 >  2:265724/1‑96 (MQ=255)                                                           gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                 <  1:265724/96‑1 (MQ=255)                                                                  ggaGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAg                                                               <  1:272725/139‑1 (MQ=255)                                                                    aGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGtt                                                             >  1:50759/1‑139 (MQ=255)                                                                         aGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                        <  2:771214/139‑1 (MQ=255)                                                                                         gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCAGGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGAAGGCATTGCTGAAGTTGCTGCCAGTCATGGACAccgg                                        >  1:71434/1‑137 (MQ=255)                                                                                         gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtg                                                            <  2:108539/119‑1 (MQ=255)                                                                                         gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtg                                                            >  1:108539/1‑119 (MQ=255)                                                                                               gCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTa                                  <  1:57827/139‑1 (MQ=255)                                                                                                             aCGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTc                    <  2:50759/139‑1 (MQ=255)                                                                                                                               ggggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCcg  >  2:462146/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                  CGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCG  >  NC_000913/1458917‑1459181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑