Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,513,468 T→C S218P (TCC→CCC)  ydcT → putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,513,4680TC100.0% 141.2 / NA 40S218P (TCC→CCC) ydcTputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

ATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCC  >  NC_000913/1513333‑1513588
                                                                                                                                       |                                                                                                                        
aTGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATcccc                                                                                                                       <  1:520089/139‑1 (MQ=255)
    aGCTGGAACTGAAAAAACTGCAGCAGTCTCTCGGTATCAGTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                   <  1:121209/139‑1 (MQ=255)
     gCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgc                                                                                                                  <  1:660079/139‑1 (MQ=255)
      cTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                 <  1:774419/139‑1 (MQ=255)
      cTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                 <  2:72784/139‑1 (MQ=255)
          aaCTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAtct                                                                                                             >  1:213188/1‑139 (MQ=255)
          aaCTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAtct                                                                                                             >  1:40944/1‑139 (MQ=255)
                                  tcGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGtt                                                                                     <  2:13128/139‑1 (MQ=255)
                                     gTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                               >  1:690642/1‑110 (MQ=255)
                                     gTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAt                                                                                                               <  2:690642/110‑1 (MQ=255)
                                        tCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                 >  2:737537/1‑105 (MQ=255)
                                        tCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                 <  1:737537/105‑1 (MQ=255)
                                         cACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGcc                                                                              <  2:40944/139‑1 (MQ=255)
                                            ttttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCggg                                                                           <  2:595696/139‑1 (MQ=255)
                                              ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                         >  1:202143/1‑139 (MQ=255)
                                                    tCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTgg                                                                   >  2:248338/1‑139 (MQ=255)
                                                     cGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGt                                                                  <  1:538983/139‑1 (MQ=255)
                                                      gTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgc                                                                                                                  <  2:56469/90‑1 (MQ=255)
                                                      gTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgc                                                                                                                  >  1:56469/1‑90 (MQ=255)
                                                         aCCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACAt                                                              >  2:55670/1‑139 (MQ=255)
                                                          cccACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATc                                                             >  1:464756/1‑139 (MQ=255)
                                                                    gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGt                                                       <  1:297228/135‑1 (MQ=255)
                                                                    gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGt                                                       >  2:297228/1‑135 (MQ=255)
                                                                     ggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTg                                                  >  2:298328/1‑139 (MQ=255)
                                                                      gCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGa                                                 >  1:799750/1‑139 (MQ=255)
                                                                       cGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                <  2:213188/139‑1 (MQ=255)
                                                                         aaGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATgg                                              <  2:147949/139‑1 (MQ=255)
                                                                                 tCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGCTCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATgg                                      <  1:510844/139‑1 (MQ=255)
                                                                                 tCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCgcgc                                                                                             >  2:498194/1‑84 (MQ=255)
                                                                                 tCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCgcgc                                                                                             <  1:498194/84‑1 (MQ=255)
                                                                                   gATGTCCGAGCGTTTGGCGGTTTTCAATAATGGCCGCATTGAGCAGGTCGATCCCCCGCTCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCa                                    <  1:271057/139‑1 (MQ=255)
                                                                                      gTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCagag                                 >  2:392698/1‑139 (MQ=255)
                                                                                               tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTtgt                        >  1:209272/1‑139 (MQ=255)
                                                                                                   gCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATgg                                              <  1:427262/113‑1 (MQ=255)
                                                                                                   gCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCa                    <  2:798425/139‑1 (MQ=255)
                                                                                                   gCGGCTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATgg                                              >  2:427262/1‑113 (MQ=255)
                                                                                                    agacagtccattatGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCAt                   <  2:699820/127‑1 (MQ=255)
                                                                                                                   gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATACGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGc    >  1:13241/1‑139 (MQ=255)
                                                                                                                     cGCATTGAGCAGGTCGTTCCCCGGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGTTACATCGAATGTTTTTGTGGGCCTGATGCCGGGGAACCTTTGTGGCATACCGGGAAGCtctgtct  >  1:699820/1‑133 (MQ=255)
                                                                                                                        aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTc      <  1:424078/132‑1 (MQ=255)
                                                                                                                        aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTGTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTc      >  2:424078/1‑132 (MQ=255)
                                                                                                                                       |                                                                                                                        
ATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCC  >  NC_000913/1513333‑1513588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: