Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,548,360 C→T D320D (GAC→GAT fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,548,3600CT100.0% 98.7 / NA 32D320D (GAC→GATfdnGformate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/14);  total (18/14)

ACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGC  >  NC_000913/1548239‑1548490
                                                                                                                         |                                                                                                                                  
aCATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTa                                                                                                                   >  2:456707/1‑139 (MQ=255)
    tACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAACACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGCGCGTGCTGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTacgac                                                                                                               >  2:534922/1‑139 (MQ=255)
          acTGTCGGGCATTTGACGCTACCTGTTCAAAAACAACAAAATCAACACCGAATACGTTAAGCATTTCACGATCGCCTGCCTACTGGTGCGTGAGTATTTTGCTTTCGAAGATGGTTTGTTCAGCGGCTACGACGCTGaa                                                                                                         <  1:768852/138‑1 (MQ=255)
              tCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAAc                                                                                                     >  2:768852/1‑139 (MQ=255)
              tCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAAc                                                                                                     <  1:298602/139‑1 (MQ=255)
                  gCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCa                                                                                                 <  2:517697/139‑1 (MQ=255)
                   cGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCaa                                                                                                >  2:201365/1‑139 (MQ=255)
                                     cGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATTAATCGTCCCgg                                                                              <  1:464539/139‑1 (MQ=255)
                                                       cGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaa                                                            >  1:88983/1‑139 (MQ=255)
                                                       cGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaa                                                            >  2:595918/1‑139 (MQ=255)
                                                         ccGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATTAGCTCGATGaaaa                                                          <  1:539956/139‑1 (MQ=255)
                                                                           acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGa                                        >  1:353000/1‑139 (MQ=255)
                                                                             acCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATg                                      <  2:503121/139‑1 (MQ=255)
                                                                               cAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTc                                                                                                                           <  1:727594/52‑1 (MQ=255)
                                                                               cAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTc                                                                                                                           >  2:727594/1‑52 (MQ=255)
                                                                                  cGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAaca                                 >  1:730176/1‑139 (MQ=255)
                                                                                    ccAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGc                                                                                                             >  1:56967/1‑61 (MQ=255)
                                                                                    ccAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGc                                                                                                             <  2:56967/61‑1 (MQ=255)
                                                                                        ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACt                           >  2:192890/1‑139 (MQ=255)
                                                                                                tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg                   <  1:595918/139‑1 (MQ=255)
                                                                                                tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg                   <  2:765347/139‑1 (MQ=255)
                                                                                                tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg                   <  1:132547/139‑1 (MQ=255)
                                                                                                  cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAAt                                                                                      <  1:265014/70‑1 (MQ=255)
                                                                                                  cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAAt                                                                                      >  2:265014/1‑70 (MQ=255)
                                                                                                  cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt                 <  1:456707/139‑1 (MQ=255)
                                                                                                  cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt                 >  1:670423/1‑139 (MQ=255)
                                                                                                    tgatgaTTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACg                                                                                            >  2:530725/1‑62 (MQ=255)
                                                                                                    tgatgaTTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACg                                                                                            <  1:530725/62‑1 (MQ=255)
                                                                                                      atgatTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCtgtgt             >  2:325136/1‑139 (MQ=255)
                                                                                                      atgatTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGGCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACGCATCCGCGCtgtgt             >  2:342561/1‑139 (MQ=255)
                                                                                                                tttCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACctg   >  2:307901/1‑139 (MQ=255)
                                                                                                                 ttCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACctgc  >  2:537532/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                                  
ACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGC  >  NC_000913/1548239‑1548490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: