Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,874,798 G→A *149* (TAG→TAA
T260I (ACT→ATT) 
yeaL →
yeaM ←
UPF0756 family putative inner membrane protein
putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,874,7980GA100.0% 122.6 / NA 40*149* (TAG→TAA
T260I (ACT→ATT) 
yeaL
yeaM
UPF0756 family putative inner membrane protein
putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/28);  total (12/28)

TAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGT  >  NC_000913/1874665‑1874902
                                                                                                                                     |                                                                                                        
tAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGtaattaat                                                                                                     <  1:592919/139‑1 (MQ=255)
  aTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCGGATTGTGGGGAAACAGTAATTAAtct                                                                                                   <  2:709809/139‑1 (MQ=255)
     ggCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGc                                                                                                <  2:215530/139‑1 (MQ=255)
         gccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAata                                                                                            >  1:366703/1‑139 (MQ=255)
                       gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                              >  1:93211/1‑139 (MQ=255)
                            cGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGcc                                                                         <  2:195269/139‑1 (MQ=255)
                                  gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                   <  1:424775/139‑1 (MQ=255)
                                  gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                   <  2:93211/139‑1 (MQ=255)
                                  gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                   <  2:632711/139‑1 (MQ=255)
                                  gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                   <  2:315086/139‑1 (MQ=255)
                                  gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                   <  2:362168/139‑1 (MQ=255)
                                  gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                   <  1:797330/139‑1 (MQ=255)
                                          gCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                           <  1:107691/139‑1 (MQ=255)
                                            aCTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                         <  2:299703/139‑1 (MQ=255)
                                              tGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                       <  2:459203/139‑1 (MQ=255)
                                               gTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                                   <  2:563371/94‑1 (MQ=255)
                                               gTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                                   >  1:563371/1‑94 (MQ=255)
                                               gTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACAt                                                      <  2:96041/139‑1 (MQ=255)
                                                ttttAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATa                                                     >  2:446839/1‑139 (MQ=255)
                                                          atggcTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGGTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAgg                                           >  1:752735/4‑139 (MQ=255)
                                                          aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAgg                                           >  2:259189/1‑139 (MQ=255)
                                                          aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAgg                                           >  2:245243/1‑139 (MQ=255)
                                                                gTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttg                                     <  2:212244/139‑1 (MQ=255)
                                                                   tCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGttgttg                                  <  1:446839/139‑1 (MQ=255)
                                                                     gcggcgTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGaa                                <  2:644766/139‑1 (MQ=255)
                                                                           tACCGGTTGGGCCGCTTATTGCCGTGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCAt                            <  2:597905/137‑1 (MQ=255)
                                                                           tACCGGTTGGGCCGCTTATTGCCGTGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCAt                            >  1:597905/1‑137 (MQ=255)
                                                                           tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGccc                                                                        <  1:799844/93‑1 (MQ=255)
                                                                           tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGccc                                                                        >  2:799844/1‑93 (MQ=255)
                                                                             ccGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                           >  1:5426/1‑104 (MQ=255)
                                                                             ccGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                           <  2:5426/104‑1 (MQ=255)
                                                                                     gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGccc                                                                        <  1:307878/83‑1 (MQ=255)
                                                                                     gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGccc                                                                        >  2:307878/1‑83 (MQ=255)
                                                                                          ttATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa           <  1:581766/139‑1 (MQ=255)
                                                                                          ttATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa           <  2:349661/139‑1 (MQ=255)
                                                                                            aTTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAAc         <  2:368773/139‑1 (MQ=255)
                                                                                            aTTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAAc         <  2:366703/139‑1 (MQ=255)
                                                                                               gCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt      <  2:363628/139‑1 (MQ=255)
                                                                                                   cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGt  <  2:752735/139‑1 (MQ=255)
                                                                                                                              aaCAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt      <  1:744631/108‑1 (MQ=255)
                                                                                                                              aaCAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt      >  2:744631/1‑108 (MQ=255)
                                                                                                                                     |                                                                                                        
TAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGT  >  NC_000913/1874665‑1874902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: