Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 177,662 C→T *267* (TAG→TAA btuF ← vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913177,6620CT100.0% 93.9 / NA 30*267* (TAG→TAAbtuFvitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/17);  total (13/17)

AAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGCTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTG  >  NC_000913/177536‑177791
                                                                                                                              |                                                                                                                                 
aaaaGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAAGCGTTAATCTACCtgt                                                                                                                       >  2:543564/1‑139 (MQ=255)
               aaTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACag                                                                                                        >  2:324841/1‑139 (MQ=255)
                           aaCAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCa                                                                                            >  2:82833/1‑139 (MQ=255)
                             cAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGc                                                                                          <  2:740537/139‑1 (MQ=255)
                                                      gCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                               >  2:792341/1‑77 (MQ=255)
                                                      gCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                               <  1:792341/77‑1 (MQ=255)
                                                          tgCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCaa                                                             >  1:11930/1‑139 (MQ=255)
                                                                  atatCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCa                                                     <  2:699265/139‑1 (MQ=255)
                                                                  atatCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCa                                                     <  2:520214/139‑1 (MQ=255)
                                                                         gCCAATAGACGAGCATTGTACAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGa                                                                                                                     >  1:735436/1‑68 (MQ=255)
                                                                         gCCAATAGACGAGCATTGTACAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGa                                                                                                                     <  2:735436/68‑1 (MQ=255)
                                                                              tAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCtg                                                                                                   >  2:378111/1‑81 (MQ=255)
                                                                              tAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCtg                                                                                                   <  1:378111/81‑1 (MQ=255)
                                                                                gACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAAt                                       <  2:611326/139‑1 (MQ=255)
                                                                                    aGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACg                                   >  1:632966/1‑139 (MQ=255)
                                                                                               aTCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg                        <  1:324841/139‑1 (MQ=255)
                                                                                               aTCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGCAATAACGGGAATTTTGAg                        <  2:527188/139‑1 (MQ=255)
                                                                                               aTCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACATGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCATGTTCATACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg                        <  2:535652/139‑1 (MQ=255)
                                                                                                  cccACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTg                     <  1:608385/139‑1 (MQ=255)
                                                                                                    cACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGtt                   <  1:102846/139‑1 (MQ=255)
                                                                                                    cACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGtt                   <  2:11930/139‑1 (MQ=255)
                                                                                                     aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAg                                                                                           >  2:461134/1‑66 (MQ=255)
                                                                                                     aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAg                                                                                           <  1:461134/66‑1 (MQ=255)
                                                                                                      cTGAGCACCTGTAAAGGTCAGGCGTTAATCAACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCGAACCAGGCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCa                 <  1:16273/139‑1 (MQ=255)
                                                                                                          gCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                                      <  1:772649/50‑1 (MQ=255)
                                                                                                          gCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                                      >  2:772649/1‑50 (MQ=255)
                                                                                                              cTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTa         >  2:330535/1‑139 (MQ=255)
                                                                                                                     ggTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTg  >  1:89802/1‑139 (MQ=255)
                                                                                                                      gTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCa                                                              >  1:753011/1‑78 (MQ=255)
                                                                                                                      gTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCa                                                              <  2:753011/78‑1 (MQ=255)
                                                                                                                              |                                                                                                                                 
AAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGCTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTG  >  NC_000913/177536‑177791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: