Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,952,661	G→A	*132* (TAG→TAA)	yecN →	MAPEG family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,952,661	0	G	A	100.0%	69.9 / NA	23	*132* (TAG→TAA)	yecN	MAPEG family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/9);  total (14/9)

TGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATA  >  NC_000913/1952525‑1952792                                                                                                                                         |                                                                                                                                     tGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAacg                                                                                                                                    <  2:661990/139‑1 (MQ=255) tGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAacg                                                                                                                                    <  2:387210/139‑1 (MQ=255)             gTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCa                                                                                                                        >  2:126463/1‑139 (MQ=255)                         gTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTcc                                                                                                            <  1:544896/139‑1 (MQ=255)                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTccc                                                                                                           >  2:505557/1‑139 (MQ=255)                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTccc                                                                                                           <  1:99320/139‑1 (MQ=255)                                 cGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGAtttt                                                                                                    <  1:108792/139‑1 (MQ=255)                                                                                gATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAg                                                    >  1:302838/1‑139 (MQ=255)                                                                                                          tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa                           >  2:63067/1‑139 (MQ=255)                                                                                                          tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAAc                          >  1:719710/1‑139 (MQ=255)                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                          >  2:191522/1‑91 (MQ=255)                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                          <  1:191522/91‑1 (MQ=255)                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgat                              >  1:51171/1‑134 (MQ=255)                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgat                              >  2:210112/1‑134 (MQ=255)                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgat                              <  1:210112/134‑1 (MQ=255)                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgat                              <  2:51171/134‑1 (MQ=255)                                                                                                              cTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGt                      <  1:126463/139‑1 (MQ=255)                                                                                                               tGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                        <  1:235460/120‑1 (MQ=255)                                                                                                               tGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                        >  2:235460/1‑120 (MQ=255)                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAg                    >  1:274891/1‑139 (MQ=255)                                                                                                                       ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                        <  2:133735/49‑1 (MQ=255)                                                                                                                       ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                        >  1:133735/1‑49 (MQ=255)                                                                                                                       ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt             >  1:561551/1‑139 (MQ=255)                                                                                                                       ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt             >  1:416625/1‑139 (MQ=255)                                                                                                                                  tGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata  >  2:329394/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     TGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTA‑TTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATA  >  NC_000913/1952525‑1952792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑