Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,139,759	C→T	*618* (TAG→TAA)	asmA ←	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,139,759	0	C	T	100.0%	150.0 / NA	47	*618* (TAG→TAA)	asmA	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

GTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGGC  >  NC_000913/2139628‑2139889                                                                                                                                    |                                                                                                                                   gtagCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACAtct                                                                                                                             <  1:115542/139‑1 (MQ=255)     cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGCCTGGTCACCTGGTAACTTACATCTtctc                                                                                                                         >  2:778142/1‑139 (MQ=255)     cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTAc                                                                                                                                 <  2:446458/131‑1 (MQ=255)     cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTAc                                                                                                                                 >  1:446458/1‑131 (MQ=255)     cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTtctc                                                                                                                         >  2:808/1‑139 (MQ=255)     cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTtctc                                                                                                                         >  2:305473/1‑139 (MQ=255)     cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTtctc                                                                                                                         >  2:197538/1‑139 (MQ=255)       aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                                       >  2:61193/1‑139 (MQ=255)            cATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  >  2:200760/1‑139 (MQ=255)            cATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                  <  1:808/139‑1 (MQ=255)                      aCGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACAt                                                                                                        >  1:560559/1‑139 (MQ=255)                         gAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCtt                                                                                                     <  1:197538/139‑1 (MQ=255)                          aaCAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                    >  2:444922/1‑139 (MQ=255)                                     aTCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCCTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         <  1:484574/139‑1 (MQ=255)                                         tACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         <  1:111672/135‑1 (MQ=255)                                         tACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         >  2:111672/1‑135 (MQ=255)                                         tACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATc                                                                                     >  2:250318/1‑139 (MQ=255)                                                tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTc                                                                              >  1:297471/1‑139 (MQ=255)                                                          atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGccc                                                                    <  2:791462/139‑1 (MQ=255)                                                              tgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTc                                                                >  1:718887/1‑139 (MQ=255)                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACa                                                                                                                                <  1:593702/73‑1 (MQ=255)                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACa                                                                                                                                >  2:593702/1‑73 (MQ=255)                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         >  2:175446/1‑112 (MQ=255)                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         <  1:267899/112‑1 (MQ=255)                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         >  2:267899/1‑112 (MQ=255)                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         <  1:175446/112‑1 (MQ=255)                                                                ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCTGCTCGGCCCAGTCAt                                                              >  1:158024/1‑139 (MQ=255)                                                                  gccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa                                                                                             >  1:375415/1‑106 (MQ=255)                                                                  gccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa                                                                                             <  2:375415/106‑1 (MQ=255)                                                                                aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGc                                              >  2:17542/1‑139 (MQ=255)                                                                                 tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                             <  1:243187/139‑1 (MQ=255)                                                                                 tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                             <  2:560559/139‑1 (MQ=255)                                                                                 tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                             <  2:356803/139‑1 (MQ=255)                                                                                    gACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                                          <  2:415551/139‑1 (MQ=255)                                                                                      cGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                                        <  2:297471/139‑1 (MQ=255)                                                                                      cGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                                        <  2:502054/139‑1 (MQ=255)                                                                                                   ttATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTGCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTg                           <  1:305473/139‑1 (MQ=255)                                                                                                     aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg                         >  2:320434/1‑139 (MQ=255)                                                                                                     aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg                         <  1:200760/139‑1 (MQ=255)                                                                                                     aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTAGTCCTGTAGATGTTTgcg                         <  1:444922/139‑1 (MQ=255)                                                                                                      tCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc                        <  1:320434/139‑1 (MQ=255)                                                                                                                    cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         >  2:363967/1‑60 (MQ=255)                                                                                                                    cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         <  1:186736/60‑1 (MQ=255)                                                                                                                    cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         >  2:186736/1‑60 (MQ=255)                                                                                                                    cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                         <  1:363967/60‑1 (MQ=255)                                                                                                                       gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTg       >  1:15386/1‑139 (MQ=255)                                                                                                                            cTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGGCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGGc  <  2:17695/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   GTAGCGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGGC  >  NC_000913/2139628‑2139889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑