Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,218,207	C→T	A117A (GCG→GCA)	yehY ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,218,207	0	C	T	100.0%	97.5 / NA	31	A117A (GCG→GCA)	yehY	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

GTGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGCGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGT  >  NC_000913/2218074‑2218336                                                                                                                                      |                                                                                                                                  gTGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGtgccgc                                                                                                                              >  1:89410/1‑139 (MQ=255)    agagtTCGTTAAGCGTGCCGAAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACATCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagc                                                                                                                           <  1:168919/139‑1 (MQ=255)       gaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagccag                                                                                                                        <  2:89410/139‑1 (MQ=255)          cGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAgccagcca                                                                                                                     <  2:603326/139‑1 (MQ=255)            ttAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGa                                                                                                                   >  1:148014/1‑139 (MQ=255)            ttAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGa                                                                                                                   >  2:39636/1‑139 (MQ=255)            ttAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGa                                                                                                                   <  1:459987/139‑1 (MQ=255)            ttAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGa                                                                                                                   >  1:599419/1‑139 (MQ=255)                  gTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCAc                                                                                                             >  1:478554/1‑139 (MQ=255)                   tGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACt                                                                                                            >  1:22535/1‑139 (MQ=255)                     ccGGAGTACAGCGACCACAGCGGAATAATGATACTCTGCATATGCAACAACCCGGGCCACAACAGATGCGTGGAGATGCGGAGGATGGCGTCCCTGCAGGCCAGCAAGGCCAGTGCCGCAGCCAGCCAGAAACCaatca                                                                                                          >  1:131179/1‑135 (MQ=255)                               gCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGt                                                                                                >  2:10942/1‑139 (MQ=255)                                               aatGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTg                                                                                <  1:622719/139‑1 (MQ=255)                                                      aTCTGCATATGCAACAACCAGCGCCACAGCGGATGCGAGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCAtttt                                                                         >  1:376020/1‑139 (MQ=255)                                                                     aacCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcg                                                          >  1:93656/1‑139 (MQ=255)                                                                        cAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcgccg                                                       >  2:421450/1‑139 (MQ=255)                                                                         aGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACCGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcgccgc                                                      >  1:141410/1‑139 (MQ=255)                                                                                 aGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                              <  2:472683/139‑1 (MQ=255)                                                                                 aGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACCGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                              <  2:141410/139‑1 (MQ=255)                                                                                           tGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGATACGCGCCAGCCCACTGCCATTTTGCACCAGTCGGGTCGCCGCCTTTCCAGCTCCCCacac                                    <  1:518039/139‑1 (MQ=255)                                                                                                    ggcggATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATAc                           <  1:39636/139‑1 (MQ=255)                                                                                                     gcggATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACg                          <  2:723523/139‑1 (MQ=255)                                                                                                               tCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAAtt                <  2:478554/139‑1 (MQ=255)                                                                                                                   tACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTgcgc            >  2:85172/1‑139 (MQ=255)                                                                                                                     cAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCa          >  2:753629/1‑139 (MQ=255)                                                                                                                           aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAAt                 <  1:712206/126‑1 (MQ=255)                                                                                                                           aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAAt                 >  2:712206/1‑126 (MQ=255)                                                                                                                           aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGa    >  2:559644/1‑139 (MQ=255)                                                                                                                           aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGa    >  1:736888/1‑139 (MQ=255)                                                                                                                           aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCACCAGAATGa    >  2:489460/1‑139 (MQ=255)                                                                                                                             cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGt  >  1:779945/1‑139 (MQ=255)                                                                                                                                  ccAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAgcg                                                                                     >  1:435386/1‑51 (MQ=255)                                                                                                                                  ccAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAgcg                                                                                     <  2:435386/51‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  GTGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGCGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGT  >  NC_000913/2218074‑2218336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑