Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,350,162	A→G	L284L (TTG→CTG)	glpQ ←	periplasmic glycerophosphodiester phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,350,162	0	A	G	100.0%	173.8 / NA	48	L284L (TTG→CTG)	glpQ	periplasmic glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/18);  total (30/18)

TGATTCACATCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAACATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCA  >  NC_000913/2350026‑2350291                                                                                                                                         |                                                                                                                                  tGATTCACATCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCa                                                                                                                                 >  2:685143/1‑139 (MQ=255)          tCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAAt                                                                                                                        >  1:164478/1‑139 (MQ=255)           cAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                       <  2:317951/139‑1 (MQ=255)                   tATTCAGGCAGTTTATCTGACCGCACGGTCTAAGGATGCACTACCAGTTTATTCTGCTGCGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCTTATGGTATTCCGTACCaa                                                                                                               >  1:124270/1‑139 (MQ=255)                   tATTCAGGAAGTTTATCTGACCGCACGGTCTAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCaa                                                                                                               >  1:323310/1‑139 (MQ=255)                    aTTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAAt                                                                                                              <  2:370931/139‑1 (MQ=255)                        aGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                       <  1:392631/126‑1 (MQ=255)                        aGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                       >  2:392631/1‑126 (MQ=255)                           cAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCt                                                                                                       >  1:464996/1‑139 (MQ=255)                            aGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTg                                                                                                      <  1:709040/139‑1 (MQ=255)                                     gACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCg                                                                                             <  2:464996/139‑1 (MQ=255)                                     gACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCg                                                                                             <  2:323310/139‑1 (MQ=255)                                      aCCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCa                                                                                                          <  2:613024/125‑1 (MQ=255)                                      aCCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCa                                                                                                          >  1:613024/1‑125 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCa                                                                                                                                 >  1:538001/1‑83 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                       <  2:653253/93‑1 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                       >  1:653253/1‑93 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAcc                                                                                        <  2:219416/124‑1 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAcc                                                                                        >  1:219416/1‑124 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  2:185485/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  1:88336/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  2:323732/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  2:520379/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  2:655053/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  1:780209/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  1:436116/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  1:639272/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  1:637537/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                         >  1:618995/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTCTTTCATGGCAccc                                                                         >  1:530933/1‑139 (MQ=255)                                                                aGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTaa                                                                  <  2:299904/139‑1 (MQ=255)                                                                    tATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACAt                                                              <  1:95638/139‑1 (MQ=255)                                                                          gctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTc                                                        <  2:88336/139‑1 (MQ=255)                                                                           ctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCg                                                       >  1:709415/1‑139 (MQ=255)                                                                           ctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATAGGGTAATCCGGACCAATACCAGCTTCATATTCCGGCAGCTGTTTCATGGCGCCCGGCGTAAACATccggacg                                                       <  1:334307/139‑6 (MQ=255)                                                                                cATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGtt                                                  <  2:428005/139‑1 (MQ=255)                                                                                   cTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTa                                               <  2:333969/139‑1 (MQ=255)                                                                                     tGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAAt                                             >  1:574564/1‑139 (MQ=255)                                                                                           aTGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAAccc                                       >  2:449509/1‑139 (MQ=255)                                                                                            tGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCa                                      >  2:407432/1‑139 (MQ=255)                                                                                             gCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAcc                                                                          <  2:723194/102‑1 (MQ=255)                                                                                             gCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAcc                                                                          >  1:723194/1‑102 (MQ=255)                                                                                             gCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAg                                     >  1:39948/1‑139 (MQ=255)                                                                                                  tGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTcc                                >  1:384356/1‑139 (MQ=255)                                                                                                              tACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGttt                    >  2:547454/1‑139 (MQ=255)                                                                                                                          aTGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATcc                                                            >  1:58136/1‑87 (MQ=255)                                                                                                                          aTGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATcc                                                            <  2:58136/87‑1 (MQ=255)                                                                                                                                cctcAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCa  <  1:547454/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  TGATTCACATCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAACATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCA  >  NC_000913/2350026‑2350291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑