Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,379,348	C→T	*432* (TAG→TAA)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,348	0	C	T	100.0%	94.2 / NA	30	*432* (TAG→TAA)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/11);  total (19/11)

AATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGA  >  NC_000913/2379218‑2379482                                                                                                                                   |                                                                                                                                       aaTGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                <  1:408442/139‑1 (MQ=255) aaTGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                <  1:639564/139‑1 (MQ=255)    gACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTg                                                                                                                             >  1:39146/1‑139 (MQ=255)     aCCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGGATCAATATTGCGGAGCAGTATAAGGATCTACAAAAATAGATTATTGATATGAATAGGTAATGATGCGACTCATTATTATTGCATTTGt                                                                                                                            >  1:447802/1‑139 (MQ=255)       cgccgcCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGtaa                                                                                                                          >  1:579882/1‑139 (MQ=255)                                 ttACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTttgt                                                                                                >  2:645775/1‑139 (MQ=255)                                          tGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGAttt                                                                                       <  2:39146/139‑1 (MQ=255)                                                  tCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCACTATTATTCCATTTGTAATAAAGTa                                                                                                                   >  1:489968/1‑103 (MQ=255)                                                  tCCGGTATCAATATTGCGGCTAAGTAAAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCAGTATTATTCCATTTGTACTAAAGTa                                                                                                                   <  2:489968/103‑1 (MQ=255)                                                       tATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTg                                                                          >  2:452439/1‑139 (MQ=255)                                                          cAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                       <  2:579882/139‑1 (MQ=255)                                                                tGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTc                                                                 >  1:223028/1‑139 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                >  2:690159/1‑139 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                <  2:312285/139‑1 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                >  1:691558/1‑139 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                >  1:312285/1‑139 (MQ=255)                                                                  cGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCgg                                                               <  1:645775/139‑1 (MQ=255)                                                                       aaGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCa                                                          <  2:651002/139‑1 (MQ=255)                                                                         gTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACg                                                        >  2:758394/1‑139 (MQ=255)                                                                                  gCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGcc                                               >  1:132149/1‑139 (MQ=255)                                                                                   cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAAGCACGGACAATGccc                                              >  1:589457/1‑139 (MQ=255)                                                                                    tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                             >  2:740425/1‑139 (MQ=255)                                                                                    tACAAAAATAGATTATTGATATGAATCGGTAATGAAGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                             >  1:575933/1‑139 (MQ=255)                                                                                       aaaaaTAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                          <  2:691558/139‑1 (MQ=255)                                                                                       aaaaaTAGATTATTGATATGAATCGGTAATGATGCAACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                          <  1:177695/139‑1 (MQ=255)                                                                                                      atatGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCa                           <  1:690159/139‑1 (MQ=255)                                                                                                                gTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGcc                 >  2:751197/1‑139 (MQ=255)                                                                                                                       tgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAAtt          >  2:297432/1‑139 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg      >  1:566926/1‑139 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg      >  1:478073/1‑139 (MQ=255)                                                                                                                               attattattCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGa  >  2:762911/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                       AATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGA  >  NC_000913/2379218‑2379482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑