Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,699	T→C	intergenic (+27/‑48)	ackA → / → pta	acetate kinase A and propionate kinase 2/phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,699	0	T	C	100.0%	146.5 / NA	41	intergenic (+27/‑48)	ackA/pta	acetate kinase A and propionate kinase 2/phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/27);  total (14/27)

TTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTG  >  NC_000913/2414565‑2414822                                                                                                                                       |                                                                                                                            ttCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTg                                                                                                                         >  2:730033/1‑139 (MQ=255)       aaaTCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGt                                                                                                                   <  1:524208/139‑1 (MQ=255)                        caaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcggcg                                                                                                  <  1:28945/139‑1 (MQ=255)                                   cccGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCTCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaaa                                                                                         <  1:571133/137‑1 (MQ=255)                                   cccGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaaa                                                                                         >  2:571133/1‑137 (MQ=255)                                   cccGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAaga                                                                                       >  2:631727/1‑139 (MQ=255)                                       tCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGAt                                                                                   >  1:220066/1‑139 (MQ=255)                                       tCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGAt                                                                                   >  1:539433/1‑139 (MQ=255)                                       tCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGAt                                                                                   <  1:428464/139‑1 (MQ=255)                                           gCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGACTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAAc                                                                               >  2:415840/1‑139 (MQ=255)                                            cGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAAcc                                                                              <  2:436812/139‑1 (MQ=255)                                              gtggtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCgt                                                                            <  1:429658/139‑1 (MQ=255)                                               tggtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCgtg                                                                           <  1:415840/139‑1 (MQ=255)                                               tggtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCgtg                                                                           <  1:498095/139‑1 (MQ=255)                                                 gtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                                         <  1:561038/139‑1 (MQ=255)                                                 gtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                                         <  1:165761/139‑1 (MQ=255)                                                    aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      <  1:730033/139‑1 (MQ=255)                                                    aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      <  2:362317/139‑1 (MQ=255)                                                    aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      <  2:532176/139‑1 (MQ=255)                                                         aaccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAAGCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtatta                                                                 <  2:398052/139‑1 (MQ=255)                                                           ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattatt                                                               >  1:323067/1‑139 (MQ=255)                                                            caacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattatta                                                              <  2:746639/139‑1 (MQ=255)                                                               cGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                                          >  2:281092/1‑108 (MQ=255)                                                               cGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                                          <  1:281092/108‑1 (MQ=255)                                                                 aagaaCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTg                                                         <  2:598407/139‑1 (MQ=255)                                                                    aaCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATc                                                      <  2:390099/139‑1 (MQ=255)                                                                         gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      <  2:282782/118‑1 (MQ=255)                                                                         gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      >  1:282782/1‑118 (MQ=255)                                                                         gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAc                                                 <  2:608980/139‑1 (MQ=255)                                                                         gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAc                                                 >  2:11537/1‑139 (MQ=255)                                                                               gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAAc                                           >  1:415036/1‑139 (MQ=255)                                                                                          gAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTc                                >  1:348620/1‑139 (MQ=255)                                                                                                         cTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc                 <  1:351238/139‑1 (MQ=255)                                                                                                         cTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc                 <  2:348620/139‑1 (MQ=255)                                                                                                         cTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc                 <  1:631727/139‑1 (MQ=255)                                                                                                         cTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGCCTGACCAGCGTCAGcc                 <  2:415036/139‑1 (MQ=255)                                                                                                                        cagctcagctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGTTAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTg  <  2:792668/139‑1 (MQ=255)                                                                                                                           ctcagctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      <  2:457621/68‑1 (MQ=255)                                                                                                                           ctcagctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      >  1:457621/1‑68 (MQ=255)                                                                                                                               gctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      <  2:119890/64‑1 (MQ=255)                                                                                                                               gctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                                      >  1:119890/1‑64 (MQ=255)                                                                                                                                       |                                                                                                                            TTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTG  >  NC_000913/2414565‑2414822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑