Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,228 C→T *274* (TAG→TAA yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2280CT100.0% 93.6 / NA 30*274* (TAG→TAAyfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

GTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAC  >  NC_000913/2449096‑2449359
                                                                                                                                    |                                                                                                                                   
gTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAAc                                                                                                                               <  1:78809/139‑1 (MQ=255)
         cgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGt                                                                                                                      <  1:670112/139‑1 (MQ=255)
          gttgttGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTa                                                                                                                     <  2:188806/139‑1 (MQ=255)
             gttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGt                                                                                                                  >  1:94430/1‑139 (MQ=255)
                            aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAg                                                                                                   >  1:328830/1‑139 (MQ=255)
                                                   aGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATActg                                                                            <  2:246441/139‑1 (MQ=255)
                                                       gCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTgg                                                                        >  1:251803/1‑139 (MQ=255)
                                                         ccTGAGGAGTTGAGCGTGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGACCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTa                                                                      <  1:372784/139‑1 (MQ=255)
                                                              ggagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAac                                                                 <  1:453688/139‑1 (MQ=255)
                                                                       cgcgTTCCTCCATGTACTGATAATGACCGGGGTGAATGCCCCTGCTTGTTCTCACTAAGGGTTACCACTCAATCAGTATGTATAGGAACCGGCATGTAGAATCGATTGGCTGCATACTGCTGGTATCAAAACGCACGAc                                                        >  1:423928/1‑139 (MQ=255)
                                                                        gcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGAcc                                                       <  2:328830/139‑1 (MQ=255)
                                                                            tGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTg                                                                                       <  2:743782/103‑1 (MQ=255)
                                                                            tGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTg                                                                                       >  1:743782/1‑103 (MQ=255)
                                                                                             aTGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAg                                                                                                   >  1:528861/1‑74 (MQ=255)
                                                                                             aTGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAg                                                                                                   <  2:528861/74‑1 (MQ=255)
                                                                                                gCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGtt                               >  1:310744/1‑139 (MQ=255)
                                                                                                    gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCAt                                                                                                                           >  1:30587/1‑43 (MQ=255)
                                                                                                    gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCAt                                                                                                                           <  2:30587/43‑1 (MQ=255)
                                                                                                    gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGt                                                                                                                      >  1:623099/1‑48 (MQ=255)
                                                                                                    gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGt                                                                                                                      <  2:623099/48‑1 (MQ=255)
                                                                                                    gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAg                           >  1:622686/1‑139 (MQ=255)
                                                                                                    gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAg                           >  1:526134/1‑139 (MQ=255)
                                                                                                              cccTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                 <  2:310744/139‑1 (MQ=255)
                                                                                                                        tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCg                                                                                                        <  2:462844/42‑1 (MQ=255)
                                                                                                                        tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCg                                                                                                        >  1:462844/1‑42 (MQ=255)
                                                                                                                        tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                              >  2:228041/1‑84 (MQ=255)
                                                                                                                        tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                              <  1:228041/84‑1 (MQ=255)
                                                                                                                             cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGt                                        >  1:338031/1‑101 (MQ=255)
                                                                                                                             cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGt                                        <  2:338031/101‑1 (MQ=255)
                                                                                                                             cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc  >  2:575524/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAC  >  NC_000913/2449096‑2449359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: