Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,525,930	C→T	*309* (TAG→TAA)	yfeR ←	transcriptional regulator of yefH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,525,930	0	C	T	100.0%	140.6 / NA	44	*309* (TAG→TAA)	yfeR	transcriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/21);  total (23/21)

ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACT  >  NC_000913/2525794‑2526057                                                                                                                                         |                                                                                                                                caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta                                                                                                                               >  2:189593‑M2/133‑139 (MQ=255) caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta                                                                                                                               >  2:442318‑M2/133‑139 (MQ=255) caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta                                                                                                                               >  1:297578‑M2/133‑139 (MQ=255)   acatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                             <  1:116464‑M2/9‑1 (MQ=255)         cacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGAt                                                                                                                       <  2:271800‑M2/15‑1 (MQ=255)           cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                     <  1:366565‑M2/17‑1 (MQ=255)              tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc                                                                                                                  >  1:366916‑M2/120‑139 (MQ=255)               actacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAAAACg                                                                                                                 >  1:162341‑M2/119‑139 (MQ=255)                      ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACgg                                                                                                                >  2:626395‑M2/112‑133 (MQ=255)                      ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACgg                                                                                                                <  1:626395‑M2/22‑1 (MQ=255)                      ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc                                                                                                          >  2:25911‑M2/112‑139 (MQ=255)                        attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACaa                                                                                                                   <  1:301931‑M2/19‑1 (MQ=255)                        attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACaa                                                                                                                   >  2:301931‑M2/110‑128 (MQ=255)                        attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt                                                                                                        >  1:57214‑M2/110‑139 (MQ=255)                                  gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                             >  2:661049‑M2/100‑124 (MQ=255)                                  gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                             <  1:661049‑M2/25‑1 (MQ=255)                                  gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                              >  1:204433‑M2/100‑139 (MQ=255)                                        cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc                                                                                                   >  2:333764‑M2/94‑128 (MQ=255)                                        cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc                                                                                                   <  1:333764‑M2/35‑1 (MQ=255)                                          gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg                                                                                      <  2:366916‑M2/48‑1 (MQ=255)                                           ttggggAAGATTTTTAACAACATGCGATTATCTACTCCATCAAATTTAACTACTGATTGATTAGTGCAGGGGCTCCCGG‑‑‑‑‑‑GA‑‑‑CACTTTATATCTGATACAACGGATCCCCTACCCGCCCAGCCATTAGCGCATTGCCCTg                                                                            <  1:765093/134‑1 (MQ=255)                                           gatgacaaactgggcgaagatttttacaggatgcgataatctactccctcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc                                                                                                                  >  1:574211‑M2/91‑110 (MQ=255)                                           gaggacaaactgggcgaagatttttacaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc                                                                                                                  <  2:574211‑M2/20‑1 (MQ=255)                                            tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                    <  1:25911‑M2/50‑1 (MQ=255)                                            tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                    <  2:204433‑M2/50‑1 (MQ=255)                                            tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                    <  2:226338‑M2/50‑1 (MQ=255)                                                    tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                            <  2:97074‑M2/58‑1 (MQ=255)                                                      ggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGt                                                                          <  1:710732‑M2/60‑1 (MQ=255)                                                      ggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGt                                                                          >  2:793127‑M2/80‑139 (MQ=255)                                                      ggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGt                                                                          >  1:590414‑M2/80‑139 (MQ=255)                                                        cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtct                                                                        <  2:145367‑M2/62‑1 (MQ=255)                                                           agatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGt                                                                     >  2:19285‑M2/75‑139 (MQ=255)                                                               ttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                                 <  1:189593‑M2/69‑1 (MQ=255)                                                                 ttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                               <  1:442318‑M2/71‑1 (MQ=255)                                                                                      ctccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCGCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg                                          <  1:545901‑M2/92‑1 (MQ=255)                                                                                           tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                     >  2:385957‑M2/43‑139 (MQ=255)                                                                                           tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                     >  2:440439‑M2/43‑139 (MQ=255)                                                                                           tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                     >  1:237181‑M2/43‑139 (MQ=255)                                                                                                           attgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                     >  2:586636‑M2/27‑139 (MQ=255)                                                                                                                  actgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                   <  2:505412‑M2/51‑1 (MQ=255)                                                                                                                  actgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                   >  1:505412‑M2/20‑70 (MQ=255)                                                                                                                         gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                    >  2:674188‑M2/13‑62 (MQ=255)                                                                                                                         gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                    <  1:674188‑M2/50‑1 (MQ=255)                                                                                                                              cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt  >  2:365966‑M2/8‑139 (MQ=255)                                                                                                                                         |                                                                                                                                ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACT  >  NC_000913/2525794‑2526057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑