Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,655,767	T→C	T198A (ACT→GCT)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,655,767	0	T	C	100.0%	78.8 / NA	23	T198A (ACT→GCT)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/9);  total (14/9)

GCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCT  >  NC_000913/2655632‑2655898                                                                                                                                        |                                                                                                                                    gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGTGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                  >  1:436149/1‑139 (MQ=255) gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                  >  2:136837/1‑139 (MQ=255)            ttGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTtaccta                                                                                                                       >  2:421207/1‑139 (MQ=255)                 cAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                  <  2:51693/139‑1 (MQ=255)                 cAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                  <  2:36722/139‑1 (MQ=255)                    tCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTATTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAc                                                                                                               >  2:715251/1‑139 (MQ=255)                      cgcgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAcgc                                                                                                             >  2:176287/1‑139 (MQ=255)                            tAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTacac                                                                                                       <  1:715251/139‑1 (MQ=255)                                                  ggTAACCGCTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCACTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                                 <  2:475569/139‑1 (MQ=255)                                                   gTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                                <  1:421207/139‑1 (MQ=255)                                                             ccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                      >  1:223695/1‑139 (MQ=255)                                                             ccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                      <  1:661208/139‑1 (MQ=255)                                                             ccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                      >  2:359359/1‑139 (MQ=255)                                                                                   cATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGaa                                                >  2:261439/1‑139 (MQ=255)                                                                                              tAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                  >  1:365376/1‑62 (MQ=255)                                                                                              tAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                  <  2:365376/62‑1 (MQ=255)                                                                                                  cGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGt                                 >  2:580827/1‑139 (MQ=255)                                                                                                   gCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTc                                >  1:518257/1‑139 (MQ=255)                                                                                                             tGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                      <  2:518257/139‑1 (MQ=255)                                                                                                                tGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                   <  2:436149/139‑1 (MQ=255)                                                                                                                   tGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGccc                >  2:333603/1‑139 (MQ=255)                                                                                                                     tAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCa              >  2:354451/1‑139 (MQ=255)                                                                                                                                 tCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCt  >  1:761724/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                    GCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCT  >  NC_000913/2655632‑2655898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑