Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,685,910	G→A	A26T (GCC→ACC)	hmp →	fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,685,910	0	G	A	100.0%	182.6 / NA	57	A26T (GCC→ACC)	hmp	fused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/29);  total (28/29)

CTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATAT  >  NC_000913/2685775‑2686037                                                                                                                                        |                                                                                                                                cTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                              >  2:741152/1‑139 (MQ=255)              gATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                                 <  2:162063/139‑1 (MQ=255)                     ttGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGttt                                                                                                          <  1:158157/139‑1 (MQ=255)                          aTACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                     <  2:2779/139‑1 (MQ=255)                          aTACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                                     <  1:741666/139‑1 (MQ=255)                            aCATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATa                                                                                                   >  2:454847/1‑139 (MQ=255)                             cATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATaa                                                                                                  >  1:393239/1‑139 (MQ=255)                                 aaTTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCa                                                                                              <  1:741152/139‑1 (MQ=255)                                  aTTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAg                                                                                             >  2:276390/1‑139 (MQ=255)                                    tAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGaa                                                                                           >  1:384182/1‑139 (MQ=255)                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                                 <  1:533494/113‑1 (MQ=255)                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                                 >  2:533494/1‑113 (MQ=255)                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCa                                                                                       <  2:393239/139‑1 (MQ=255)                                             aaaaaaGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGaa                                                                                  >  2:120444/1‑139 (MQ=255)                                                    gAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTa                                                                           >  1:716223/1‑139 (MQ=255)                                                     aaGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                          <  1:335343/139‑1 (MQ=255)                                                     aaGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                                          <  2:633260/139‑1 (MQ=255)                                                      aGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                                   >  2:668315/1‑97 (MQ=255)                                                      aGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                                   <  1:668315/97‑1 (MQ=255)                                                        aCCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACAt                                                                       >  1:575578/1‑139 (MQ=255)                                                               gCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                                <  1:276390/139‑1 (MQ=255)                                                               gCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                                >  2:260668/1‑139 (MQ=255)                                                                  tGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGt                                                                                                                >  1:701094/1‑88 (MQ=255)                                                                  tGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGt                                                                                                                <  2:701094/88‑1 (MQ=255)                                                                   gACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGc                                                            >  2:537863/1‑139 (MQ=255)                                                                    aCGCTCAAACAATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCg                                                           <  1:674514/139‑1 (MQ=255)                                                                                tCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCa                                               <  1:643497/139‑1 (MQ=255)                                                                                          aaaaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGcc                                     <  2:575578/139‑1 (MQ=255)                                                                                           aaaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGttt                                                                                                          <  1:782634/69‑1 (MQ=255)                                                                                           aaaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGttt                                                                                                          >  2:782634/1‑69 (MQ=255)                                                                                            aaGTCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                           <  1:521195/67‑1 (MQ=255)                                                                                            aaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                           >  2:521195/1‑67 (MQ=255)                                                                                                 accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                                <  1:316036/105‑1 (MQ=255)                                                                                                 accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                                >  2:316036/1‑105 (MQ=255)                                                                                                 accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGcc                                     <  1:187874/132‑1 (MQ=255)                                                                                                 accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGcc                                     >  2:187874/1‑132 (MQ=255)                                                                                                  ccaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                              >  2:247269/1‑42 (MQ=255)                                                                                                  ccaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                              <  1:247269/42‑1 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                                 >  2:315531/1‑54 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                                 <  1:315531/54‑1 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGt                                                                                                            <  2:92247/59‑1 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGt                                                                                                            >  1:92247/1‑59 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                                >  2:420529/1‑103 (MQ=255)                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                                <  1:420529/103‑1 (MQ=255)                                                                                                               ggtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTgccgcc                <  2:308469/139‑1 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt               >  1:6503/1‑139 (MQ=255)                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt               >  2:717481/1‑139 (MQ=255)                                                                                                                 tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTa              >  1:296741/1‑139 (MQ=255)                                                                                                                  ggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGttt                               >  1:263767/1‑121 (MQ=255)                                                                                                                  ggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGttt                               <  2:263767/121‑1 (MQ=255)                                                                                                                   gAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCg                                                   <  1:685313/100‑1 (MQ=255)                                                                                                                   gAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCg                                                   >  2:685313/1‑100 (MQ=255)                                                                                                                    aaaCGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGc           <  1:454847/139‑1 (MQ=255)                                                                                                                          ggCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGcc                                     <  1:761805/107‑1 (MQ=255)                                                                                                                          ggCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGcc                                     >  2:761805/1‑107 (MQ=255)                                                                                                                          ggCCAAAGTTAACCACCCATTTCTACGACCGTATGGGTACTCATAACCCAGAACTCAAAGAAATTTCTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTTCCGCCTACGCCAgcca     >  1:214344/1‑136 (MQ=255)                                                                                                                             cAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAatat  <  1:260668/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                CTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATAT  >  NC_000913/2685775‑2686037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑