Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,786,729 G→A pseudogene (333/2253 nt) ygaQ → uncharacterized protein, putative enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,786,7290GA100.0% 220.3 / NA 67pseudogene (333/2253 nt)ygaQuncharacterized protein, putative enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/41);  total (26/41)

AAAGTTATTAATGATCTGTTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAA  >  NC_000913/2786598‑2786863
                                                                                                                                   |                                                                                                                                      
aaaGTTATTAATGATCTGTTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATc                                                                                                                                 <  1:802496/139‑1 (MQ=255)
    ttattaATGATCTGTTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACg                                                                                                                             <  1:391547/139‑1 (MQ=255)
         aaTGATCTGTTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATa                                                                                                                        <  1:440983/139‑1 (MQ=255)
          aTGATCTGTTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATaa                                                                                                                       <  1:64356/139‑2 (MQ=255)
              tCTGTTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGa                                                                                                                  >  1:423262/1‑139 (MQ=255)
                  ttAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGa                                                                                                              >  2:132082/1‑139 (MQ=255)
                       aaaCGATTATAAAATTTCCCTTTAGCCAGATACAACATACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAagag                                                                                                         <  1:385161/139‑1 (MQ=255)
                         aCGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGt                                                                                                       <  1:25144/139‑1 (MQ=255)
                                 aaaaTTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAg                                                                                               <  1:547811/139‑1 (MQ=255)
                                 aaaaTTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAg                                                                                               <  2:478601/139‑1 (MQ=255)
                                   aaTTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCg                                                                                             <  2:738341/139‑1 (MQ=255)
                                   aaTTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCg                                                                                             <  1:1488/139‑1 (MQ=255)
                                     tttCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGgt                                                                                           >  1:28447/1‑139 (MQ=255)
                                         ccTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGttt                                                                                       <  2:423262/139‑1 (MQ=255)
                                         ccTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGttt                                                                                       <  2:207750/139‑1 (MQ=255)
                                         ccTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGttt                                                                                       <  1:597960/139‑1 (MQ=255)
                                           tttCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTAt                                                                                     <  2:6035/139‑1 (MQ=255)
                                             tctcCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCa                                                                                   >  1:662908/1‑139 (MQ=255)
                                               tcCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGt                                                                                 >  2:294187/1‑139 (MQ=255)
                                                ccAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTa                                                                                <  1:94148/139‑1 (MQ=255)
                                                   gATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATgg                                                                             <  2:206026/139‑1 (MQ=255)
                                                              ccGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAAc                                                                  >  1:513311/1‑139 (MQ=255)
                                                               cGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACa                                                                 >  1:613014/1‑139 (MQ=255)
                                                                    gTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCa                                                            >  1:465914/1‑139 (MQ=255)
                                                                     ttGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCAc                                                           >  2:101652/1‑139 (MQ=255)
                                                                     ttGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCAc                                                           <  2:613014/139‑1 (MQ=255)
                                                                      tGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCAcc                                                          <  1:294187/139‑1 (MQ=255)
                                                                      tGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCAcc                                                          <  2:666744/139‑1 (MQ=255)
                                                                                    caGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGa                                            <  2:28447/139‑1 (MQ=255)
                                                                                      gCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACa                                          <  2:125707/139‑1 (MQ=255)
                                                                                       cGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATaaatg                                                                                                                    <  1:783004/65‑5 (MQ=255)
                                                                                       cGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATaaatg                                                                                                                    >  2:783004/1‑61 (MQ=255)
                                                                                       cGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAg                                                                                               <  1:726343/85‑1 (MQ=255)
                                                                                       cGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAg                                                                                               >  2:726343/1‑85 (MQ=255)
                                                                                       cGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACaa                                         >  2:549808/1‑139 (MQ=255)
                                                                                          aGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCAcc                                                          >  1:333684/1‑119 (MQ=255)
                                                                                          aGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCAcc                                                          <  2:333684/119‑1 (MQ=255)
                                                                                                aCAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTcccc                                <  1:132082/139‑1 (MQ=255)
                                                                                                 cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGaaa                                                                                                            <  1:138915/62‑1 (MQ=255)
                                                                                                 cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGaaa                                                                                                            >  2:138915/1‑62 (MQ=255)
                                                                                                 cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCa                               >  2:82276/1‑139 (MQ=255)
                                                                                                 cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCa                               >  1:249511/1‑139 (MQ=255)
                                                                                                 cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCa                               >  2:24490/1‑139 (MQ=255)
                                                                                                   gcgcCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaaa                             <  1:375561/139‑1 (MQ=255)
                                                                                                    cgcCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaaaa                            >  2:488197/1‑139 (MQ=255)
                                                                                                    cgcCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACACACATTATATCTGACAAACCTTCCCCaaaa                            <  2:129889/139‑1 (MQ=255)
                                                                                                     gcCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAg                           >  2:171069/1‑139 (MQ=255)
                                                                                                      ccAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGa                          >  2:739531/1‑139 (MQ=255)
                                                                                                         gACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGACAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATaa                       <  2:529135/139‑1 (MQ=255)
                                                                                                         gACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATaa                       <  2:275820/139‑1 (MQ=255)
                                                                                                               aTAGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGt                 <  1:101652/139‑1 (MQ=255)
                                                                                                                 aGGAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTg               >  2:484890/1‑139 (MQ=255)
                                                                                                                  ggAGAAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGc              <  1:484890/139‑1 (MQ=255)
                                                                                                                   gagaAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCa             >  1:605220/1‑139 (MQ=255)
                                                                                                                   gagaAAGAAAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCa             >  2:47071/1‑139 (MQ=255)
                                                                                                                    agaaagaaAAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCat            <  1:24490/139‑1 (MQ=255)
                                                                                                                     gaaagaaaAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCata           <  1:171069/139‑1 (MQ=255)
                                                                                                                      aaagaaaAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCatat          <  2:662908/139‑1 (MQ=255)
                                                                                                                        agaaaAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaaa                             >  1:791347/1‑118 (MQ=255)
                                                                                                                        agaaaAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaaa                             <  2:791347/118‑1 (MQ=255)
                                                                                                                        agaaaAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCat            >  1:200774/1‑135 (MQ=255)
                                                                                                                        agaaaAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCat            <  2:200774/135‑1 (MQ=255)
                                                                                                                         gaaaAACATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCt       <  1:176864/139‑1 (MQ=255)
                                                                                                                             aaCATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTa   <  2:573137/139‑1 (MQ=255)
                                                                                                                              aCATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATaa                       >  2:610843/1‑118 (MQ=255)
                                                                                                                              aCATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATaa                       <  1:610843/118‑1 (MQ=255)
                                                                                                                              aCATAAGTTTATCAACGAGATAAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTaa  <  1:64915/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
AAAGTTATTAATGATCTGTTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAA  >  NC_000913/2786598‑2786863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: