Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,859,538	C→T	L816L (CTG→TTG)	mutS →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,859,538	0	C	T	100.0%	100.5 / NA	32	L816L (CTG→TTG)	mutS	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

TACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAG  >  NC_000913/2859403‑2859670                                                                                                                                        |                                                                                                                                     tACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTtgttgt                                                                                                                                   <  2:562228/139‑1 (MQ=255)                              cGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTc                                                                                                      >  2:156031/1‑139 (MQ=255)                                tGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGa                                                                                                    >  1:348857/1‑139 (MQ=255)                                  ccAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAg                                                                                                  <  2:66787/139‑1 (MQ=255)                                           gTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGaaa                                                                                         >  1:16691/1‑139 (MQ=255)                                                    cgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                >  2:217998/1‑139 (MQ=255)                                                    cgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                >  2:179850/1‑139 (MQ=255)                                                     gcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCCGGAAAATCTTGATcc                                                                               >  2:445806/1‑139 (MQ=255)                                                      cgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCg                                                                              >  1:387797/1‑139 (MQ=255)                                                      cgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACAAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCg                                                                              >  1:386488/1‑139 (MQ=255)                                                        caaggCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCTAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGTTCTGGAAAATCTTGATCCGGa                                                                            >  1:465014/4‑139 (MQ=255)                                                            gCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtca                                                                        >  2:411663/1‑139 (MQ=255)                                                                              ggAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGc                                                                                                              >  1:278716/1‑83 (MQ=255)                                                                              ggAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGc                                                                                                              <  2:278716/83‑1 (MQ=255)                                                                                 aaGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTg                                                   <  1:217998/139‑1 (MQ=255)                                                                                              aaCGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATc                                      <  2:387797/139‑1 (MQ=255)                                                                                                     ccgcTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGaa                               <  2:279443/139‑1 (MQ=255)                                                                                                     ccgcTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGaa                               <  2:521716/139‑1 (MQ=255)                                                                                                         tACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGc                           <  1:258600/139‑1 (MQ=255)                                                                                                          aCGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGcc                          <  1:156031/139‑1 (MQ=255)                                                                                                           cGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGTAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCt                         <  2:498933/139‑1 (MQ=255)                                                                                                           cGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGa                                                                            <  2:711981/88‑1 (MQ=255)                                                                                                           cGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGa                                                                            >  1:711981/1‑88 (MQ=255)                                                                                                           cGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCt                         <  2:802633/139‑1 (MQ=255)                                                                                                           cGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCt                         <  1:179850/139‑1 (MQ=255)                                                                                                                          cGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAAtt          >  1:683087/1‑139 (MQ=255)                                                                                                                               aTGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGa     >  2:541827/1‑139 (MQ=255)                                                                                                                                tGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGAt    <  2:683087/139‑1 (MQ=255)                                                                                                                                  tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                >  2:500885/1‑61 (MQ=255)                                                                                                                                  tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                <  1:500885/61‑1 (MQ=255)                                                                                                                                  tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAAt           <  1:372910/130‑1 (MQ=255)                                                                                                                                  tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAAt           >  2:372910/1‑130 (MQ=255)                                                                                                                                  tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAg  >  2:781217/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     TACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAG  >  NC_000913/2859403‑2859670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑