Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,532	T→C	intergenic (‑114/‑25)	queE ← / → yqcG	7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,532	0	T	C	100.0%	138.4 / NA	39	intergenic (‑114/‑25)	queE/yqcG	7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/21);  total (18/21)

CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCG  >  NC_000913/2905398‑2905665                                                                                                                                       |                                                                                                                                      ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata                                                                                                                                   >  2:77004/1‑139 (MQ=255) ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata                                                                                                                                   >  2:759238/1‑139 (MQ=255) ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata                                                                                                                                   >  1:315308/1‑139 (MQ=255) ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata                                                                                                                                   >  2:593576/1‑139 (MQ=255)   cGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCaataat                                                                                                                                 >  2:258350/1‑139 (MQ=255)         tCGGGTACTGCATAGCATTTTCTGTGAAGTGGATAATTGTTAATTATTGCAGTTCCTGCCACAACAATTATGTCTTATTAGCATTCTGTTACAGGCAGGTTTAAGGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                           <  1:33526/139‑1 (MQ=255)           gggTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                                                         <  1:700376/139‑1 (MQ=255)           gggTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGa                                                                                                                         <  1:593576/139‑1 (MQ=255)             gTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                                       <  2:567135/139‑1 (MQ=255)               aCTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa                                                                                                                     <  1:701174/139‑1 (MQ=255)                           tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGTCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGCAACGTATTATGTCa                                                                                                         >  2:489841/1‑139 (MQ=255)                           tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCACTAATTAAAGGGAGTaa                                                                                                                     >  2:480048/1‑127 (MQ=255)                           tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaa                                                                                                                              >  2:340660/1‑118 (MQ=255)                           tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaa                                                                                                                              <  1:340660/118‑1 (MQ=255)                           tctcTGTGAAGTGGATAATTGTTAATTATAGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGtta                                                                                                                     <  1:480048/127‑3 (MQ=255)                             tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                           >  1:428938/1‑119 (MQ=255)                             tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                                           <  2:428938/119‑1 (MQ=255)                             tctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCaga                                                                                                       >  2:441010/1‑139 (MQ=255)                                      tGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATaa                                                                                              >  2:181267/1‑139 (MQ=255)                                           aaTTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaa                                                                                         >  1:713402/1‑139 (MQ=255)                                             ttGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAAt                                                                                       <  1:268869/139‑1 (MQ=255)                                             ttGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAAt                                                                                       <  2:315308/139‑1 (MQ=255)                                                        tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCa                                                                            <  2:343199/139‑1 (MQ=255)                                                        tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCa                                                                            <  1:615076/139‑1 (MQ=255)                                                                  gCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                                                              >  2:379867/1‑127 (MQ=255)                                                                  gCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                                                              <  1:379867/127‑1 (MQ=255)                                                                    cacaACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACAtt                                                                <  1:489841/139‑1 (MQ=255)                                                                      caacaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                                                              <  2:623638/123‑1 (MQ=255)                                                                      caacaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                                                              >  1:623638/1‑123 (MQ=255)                                                                       aacaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAGAATGGATTTAATCATGTCAAAACATTCAc                                                             >  2:752322/1‑139 (MQ=255)                                                                             cATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGACAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAAt                                                       >  2:317497/1‑139 (MQ=255)                                                                                 tCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATtata                                                   <  2:599791/139‑1 (MQ=255)                                                                                 tCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATtata                                                   <  2:66063/139‑1 (MQ=255)                                                                                 tCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATtata                                                   >  2:798945/1‑139 (MQ=255)                                                                                               tGTTACAGGCGGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGt                                     >  2:184747/1‑139 (MQ=255)                                                                                                gTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                             >  2:114514/1‑50 (MQ=255)                                                                                                gTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTaaa                                                                                                                             <  1:114514/50‑1 (MQ=255)                                                                                                 ttACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTAt                                   >  1:45179/1‑139 (MQ=255)                                                                                                       gcaggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACa                                                                  >  2:128172/1‑102 (MQ=255)                                                                                                       gcaggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACa                                                                  <  1:128172/102‑1 (MQ=255)                                                                                                               aaaGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGAt                     <  2:492392/139‑1 (MQ=255)                                                                                                                   aaaaaTGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACg                 <  1:752322/139‑1 (MQ=255)                                                                                                                                  atCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGCAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAGAATTATATTTATTTTTTCTGTATTAGTATTTAATGATAACGAATATAAAATTaata  >  1:284758/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                      CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCG  >  NC_000913/2905398‑2905665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑