Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,014,287 C→T *257* (TAG→TAA yqeC ← putative selenium‑dependent hydroxylase accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,014,2870CT100.0% 136.5 / NA 43*257* (TAG→TAAyqeCputative selenium‑dependent hydroxylase accessory protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/23);  total (20/23)

TCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAAC  >  NC_000913/3014155‑3014417
                                                                                                                                    |                                                                                                                                  
tCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTa                                                                                                                                  >  2:540759/1‑134 (MQ=255)
     ccTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAGAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAAt                                                                                                                        <  2:377137/139‑1 (MQ=255)
        cGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTg                                                                                                                     >  1:774132/1‑139 (MQ=255)
            tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                                                 <  2:236791/139‑1 (MQ=255)
                    aTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                                                         <  1:182679/139‑1 (MQ=255)
                      ttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                                       <  2:567360/139‑1 (MQ=255)
                      ttGCGCCATAGCAAACGGACAATTTTACTCTTCGAGTATTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                                       <  1:510482/139‑1 (MQ=255)
                       tGCGCCATAGCAAACGGACAGTTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                      <  2:311410/139‑1 (MQ=255)
                       tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTCACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                      >  1:759480/1‑139 (MQ=255)
                       tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                      <  2:171820/139‑1 (MQ=255)
                        gcgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt                                                                                                     >  1:420527/1‑139 (MQ=255)
                         cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                                                    <  2:774132/139‑1 (MQ=255)
                         cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                                                    <  2:420527/139‑1 (MQ=255)
                         cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                                                    <  1:774354/139‑1 (MQ=255)
                                        aCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGcc                                                                                     >  2:239958/1‑139 (MQ=255)
                                            ttttGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCccagc                                                                                 <  1:116289/139‑1 (MQ=255)
                                                            ttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATAGATATCTTTCAGTAACCCGGAATTCCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGAAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgt                                                                 <  1:290639/139‑1 (MQ=255)
                                                              gCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                                               <  1:450628/139‑1 (MQ=255)
                                                                      tgtgAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                                            >  1:454708/1‑118 (MQ=255)
                                                                      tgtgAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                                            <  2:454708/118‑1 (MQ=255)
                                                                        tgAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGcc                                                                        >  1:44872/1‑120 (MQ=255)
                                                                        tgAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGcc                                                                        <  2:44872/120‑1 (MQ=255)
                                                                        tgAAAATATTCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                                                     <  1:140519/139‑1 (MQ=255)
                                                                                ttCATACCAACCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGa                                             >  1:158314/1‑139 (MQ=255)
                                                                                        aaCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                      <  1:434716/74‑1 (MQ=255)
                                                                                        aaCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                                      >  2:434716/1‑74 (MQ=255)
                                                                                         aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg                                                                                                           <  1:341484/68‑1 (MQ=255)
                                                                                         aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg                                                                                                           >  2:341484/1‑68 (MQ=255)
                                                                                         aCCTCAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg                                    >  1:358202/1‑139 (MQ=255)
                                                                                             cAATGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCttt                                >  1:719997/1‑139 (MQ=255)
                                                                                              aaTGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtg                               >  1:413224/1‑139 (MQ=255)
                                                                                               aTGGATATCTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtgt                              >  1:110258/1‑139 (MQ=255)
                                                                                                       tcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCg                       <  1:105080/138‑1 (MQ=255)
                                                                                                       tcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCg                       <  2:110258/138‑1 (MQ=255)
                                                                                                        cTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCctct                                                                     >  2:357193/1‑92 (MQ=255)
                                                                                                        cTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCctct                                                                     <  1:357193/92‑1 (MQ=255)
                                                                                                           tCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCAtt                   >  2:111620/1‑139 (MQ=255)
                                                                                                                  cccGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                                            <  1:269412/75‑1 (MQ=255)
                                                                                                                  cccGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                                            >  2:269412/1‑75 (MQ=255)
                                                                                                                  cccGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACAt            >  2:428140/1‑139 (MQ=255)
                                                                                                                  cccGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACAt            >  2:57295/1‑139 (MQ=255)
                                                                                                                          aCCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGaaaa    <  2:759480/139‑1 (MQ=255)
                                                                                                                            ccGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAAc  >  1:417078/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                  
TCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCGCATTCTCACATTGAGAAAAAC  >  NC_000913/3014155‑3014417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: