Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,176,721 G→A T38I (ACC→ATC)  cpdA ← 3',5' cAMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,176,7210GA100.0% 120.1 / NA 38T38I (ACC→ATC) cpdA3',5' cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/21);  total (17/21)

CTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTT  >  NC_000913/3176586‑3176844
                                                                                                                                       |                                                                                                                           
cTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGatgt                                                                                                                          <  2:660023/139‑1 (MQ=255)
       tGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTAcccc                                                                                                                   >  2:656987/1‑139 (MQ=255)
               gCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAggg                                                                                                           <  1:782264/139‑1 (MQ=255)
                     tGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGt                                                                                                     >  2:143117/1‑139 (MQ=255)
                             ggCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                             <  2:700873/139‑1 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGc                                                                                           >  1:643355/1‑121 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGc                                                                                           <  2:643355/121‑1 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCTAACAGGTGAGTGTCGGt                                                                         >  2:101722/1‑139 (MQ=255)
                                                 gcgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGt                                                                         >  1:459205/1‑139 (MQ=255)
                                                  cgcTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTa                                                                        >  1:169156/1‑139 (MQ=255)
                                                      aaaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAAttt                                                                    >  1:672731/1‑139 (MQ=255)
                                                       aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACa                                                                                                              >  2:599303/1‑96 (MQ=255)
                                                       aaTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACa                                                                                                              <  1:599303/96‑1 (MQ=255)
                                                        aTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGt                                                                  >  1:205118/1‑139 (MQ=255)
                                                         tCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTa                                                                 <  2:385385/139‑1 (MQ=255)
                                                         tCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTa                                                                 <  1:143117/139‑1 (MQ=255)
                                                            ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCttt                                                                                                >  2:606824/1‑105 (MQ=255)
                                                            ccTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCttt                                                                                                <  1:606824/105‑1 (MQ=255)
                                                                             gTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCa                                             <  2:125715/139‑1 (MQ=255)
                                                                             gTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCa                                             <  1:783109/139‑1 (MQ=255)
                                                                              tCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCTAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                            <  1:101722/139‑1 (MQ=255)
                                                                              tCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                            <  1:138168/139‑1 (MQ=255)
                                                                              tCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                            <  2:459205/139‑1 (MQ=255)
                                                                                    tCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagcca                                      <  1:229482/139‑1 (MQ=255)
                                                                                       tGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCagag                                   >  2:144466/1‑139 (MQ=255)
                                                                                       agCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTATCCCAGATGTTTACCCCTAACAGGGCTTCGTACTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCAGGCCTCACCAGCCagag                                   >  2:491359/2‑139 (MQ=255)
                                                                                            gtgtGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCg                                                                                                      >  2:575793/1‑67 (MQ=255)
                                                                                            gtgtGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCg                                                                                                      <  1:575793/67‑1 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                            <  2:236970/120‑1 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                            >  1:62458/1‑120 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                            <  2:62458/120‑1 (MQ=255)
                                                                                                 gCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAc                                            >  1:236970/1‑120 (MQ=255)
                                                                                                     aaTCGCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAgg                     <  2:261945/139‑1 (MQ=255)
                                                                                                         gCCTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCttt                 <  2:563727/139‑1 (MQ=255)
                                                                                                           cTCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTcc               <  2:205118/139‑1 (MQ=255)
                                                                                                            tCCAGCACCGCCTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCa              >  2:157436/1‑139 (MQ=255)
                                                                                                                      ccTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                             >  2:461854/1‑50 (MQ=255)
                                                                                                                      ccTGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                             <  1:461854/50‑1 (MQ=255)
                                                                                                                        tGGTAACTCTCCCAGATGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCtt  <  1:491359/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                           
CTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTT  >  NC_000913/3176586‑3176844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑