Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,248,939 T→C intergenic (+117/‑30) mzrA → / → yqjC modulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,248,9390TC97.4% 278.6 / ‑0.8 78intergenic (+117/‑30)mzrA/yqjCmodulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (38/38);  minor base . (1/1);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

ATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAG  >  NC_000913/3248809‑3249067
                                                                                                                                  |                                                                                                                                
aTCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGa                                                                                                                          >  1:433461/1‑139 (MQ=255)
aTCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGa                                                                                                                          >  1:405666/1‑139 (MQ=255)
  cGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                        <  2:444266/139‑1 (MQ=255)
      tCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAGTCGTTCCTTTGTAACAGGTCCGAATc                                                                                                                       >  2:542076/1‑136 (MQ=255)
      tCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATTGTTCCTTTGTAACAGGTCCGAATc                                                                                                                       <  1:542076/136‑1 (MQ=255)
       cGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACaa                                                                                                                   >  1:800071/1‑139 (MQ=255)
              cTTCCTGTTATGCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTAGCGCTTCGTTGTCTGGACAATCGTTCGTTGGTAACAGGTCCGAATCACAatggatgg                                                                                                            >  1:481118/1‑139 (MQ=255)
                 ccTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                         <  2:593807/139‑1 (MQ=255)
                 ccTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                         >  1:652265/1‑139 (MQ=255)
                   tGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTCACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                       <  2:405666/139‑1 (MQ=255)
                   tGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                       >  2:536460/1‑139 (MQ=255)
                     ttATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGa                                                                                                     <  1:788710/139‑1 (MQ=255)
                        tCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGaa                                                                                                              >  2:524752/1‑127 (MQ=255)
                        tCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGaa                                                                                                              <  1:524752/127‑1 (MQ=255)
                        tCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATg                                                                                                  >  1:99738/1‑139 (MQ=255)
                         ccGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGa                                                                                                 >  2:182640/1‑139 (MQ=255)
                                  atCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        >  2:636085/1‑139 (MQ=255)
                                   tcactcacTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                       <  2:99738/139‑1 (MQ=255)
                                    cactcactTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATc                                                                                      >  2:654249/1‑139 (MQ=255)
                                         actTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCttt                                                                                 >  2:494571/1‑139 (MQ=255)
                                             tGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCt                                                                             >  2:587786/1‑139 (MQ=255)
                                              ggTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               >  2:485650/1‑104 (MQ=255)
                                              ggTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               <  1:485650/104‑1 (MQ=255)
                                                   tttCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                       <  2:85205/123‑1 (MQ=255)
                                                   tttCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                       >  1:85205/1‑123 (MQ=255)
                                                     tCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtctct                                                                     >  2:505250/1‑139 (MQ=255)
                                                       aCCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTctctct                                                                   >  1:713807/1‑139 (MQ=255)
                                                               tGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCtt                                                           <  1:353033/139‑1 (MQ=255)
                                                                 tCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAg                                                         <  1:199031/139‑1 (MQ=255)
                                                                 tCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAg                                                         >  1:123838/1‑139 (MQ=255)
                                                                     tGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAgg                                                                                                            <  1:447767/84‑1 (MQ=255)
                                                                     tGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAgg                                                                                                            >  2:447767/1‑84 (MQ=255)
                                                                      gATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                    <  1:536460/139‑1 (MQ=255)
                                                                      gATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCGTTGCTCTTAGTGCCg                                                    >  2:531699/1‑139 (MQ=255)
                                                                        ttAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGt                                                  <  2:304141/139‑1 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               >  1:218120/1‑75 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               <  2:218120/75‑1 (MQ=255)
                                                                             gAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTa                                             <  1:687933/139‑1 (MQ=255)
                                                                             gAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTa                                             <  2:800071/139‑1 (MQ=255)
                                                                             gAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTa                                             <  2:109028/139‑1 (MQ=255)
                                                                             gAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTa                                             <  2:652265/139‑1 (MQ=255)
                                                                             gAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTa                                             <  2:123838/139‑1 (MQ=255)
                                                                               ggCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATg                                           >  1:116413/1‑139 (MQ=255)
                                                                                gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGc                                          >  2:696125/1‑139 (MQ=255)
                                                                                       ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  2:281126/139‑1 (MQ=255)
                                                                                       ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  2:45731/139‑1 (MQ=255)
                                                                                         aaCACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCt                                 <  1:636085/139‑1 (MQ=255)
                                                                                         aaCACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCt                                 >  2:371070/1‑139 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               <  2:408971/54‑1 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               >  1:408971/1‑54 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        <  2:555397/77‑1 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        >  2:61057/1‑77 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        <  1:361527/77‑1 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        >  2:361527/1‑77 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        <  1:61057/77‑1 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        >  1:555397/1‑77 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtctc                                                                      <  2:337242/95‑1 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtctc                                                                      >  1:337242/1‑95 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  1:247485/130‑1 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   >  2:247485/1‑130 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                          >  1:26076/1‑139 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                          >  2:751424/1‑139 (MQ=255)
                                                                                                   cTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGaa                       >  2:548638/1‑139 (MQ=255)
                                                                                                     tCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGaaaa                     >  2:279835/1‑139 (MQ=255)
                                                                                                       gTTGTCTGGACAATCGTTCCTTTGTA‑‑ACGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATg                                           >  1:165517/1‑113 (MQ=255)
                                                                                                       gTTGTCTGGACAATCGTTCCTTTGTA‑‑ACGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATg                                           <  2:165517/113‑1 (MQ=255)
                                                                                                         tGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAg                 <  2:220402/139‑1 (MQ=255)
                                                                                                         tGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAg                 <  1:696125/139‑1 (MQ=255)
                                                                                                              ggACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa            <  2:300711/139‑1 (MQ=255)
                                                                                                              ggACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa            <  1:654249/139‑1 (MQ=255)
                                                                                                              ggACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa            <  2:664370/139‑1 (MQ=255)
                                                                                                                     cGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                        >  2:321529/1‑72 (MQ=255)
                                                                                                                     cGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                        <  1:321529/72‑1 (MQ=255)
                                                                                                                      gTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTa    <  1:305551/139‑1 (MQ=255)
                                                                                                                       ttCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTaa   >  2:116954/1‑139 (MQ=255)
                                                                                                                        tCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAg  <  1:505250/139‑1 (MQ=255)
                                                                                                                         ccTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   >  2:375635/1‑105 (MQ=255)
                                                                                                                         ccTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  1:375635/105‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                
ATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAG  >  NC_000913/3248809‑3249067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: