Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,299,999	G→A	A12T (GCC→ACC)	yhbQ →	GIY‑YIG nuclease superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,999	0	G	A	100.0%	97.7 / NA	31	A12T (GCC→ACC)	yhbQ	GIY‑YIG nuclease superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/10);  total (21/10)

ACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATC  >  NC_000913/3299866‑3300134                                                                                                                                      |                                                                                                                                        acaacaTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGAc                                                                                                                                    >  2:214126/1‑139 (MQ=255)         ttgttTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                            >  2:554044/1‑139 (MQ=255)               tGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAc                                                                                                                      >  1:287379/1‑139 (MQ=255)                                             ttCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTa                                                                                        <  2:287379/139‑1 (MQ=255)                                               ccATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATc                                                                                      <  1:554044/139‑1 (MQ=255)                                                  ttGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcagc                                                                                   <  1:759366/139‑1 (MQ=255)                                                                     tgtgGGCCTGACCTTAACATGCGACGCAGGCGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 <  1:563971/74‑1 (MQ=255)                                                                     tgtgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 >  2:563971/1‑74 (MQ=255)                                                                     tgtgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                                >  2:69616/1‑107 (MQ=255)                                                                     tgtgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                                <  1:69616/107‑1 (MQ=255)                                                                     tgtgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgg                                                                >  1:667754/1‑139 (MQ=255)                                                                       tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg                                                                                                                  >  2:323902/1‑87 (MQ=255)                                                                       tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg                                                                                                                  <  1:323902/87‑1 (MQ=255)                                                                       tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                              >  2:401993/1‑139 (MQ=255)                                                                       tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                              >  1:540100/1‑139 (MQ=255)                                                                       tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                              <  1:468244/139‑1 (MQ=255)                                                                       tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                              >  1:236091/1‑139 (MQ=255)                                                                         ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCg                                                            >  1:39600/1‑139 (MQ=255)                                                                         ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCg                                                            <  1:25869/139‑1 (MQ=255)                                                                         ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGAAGCACCAAAGCGGCAAAGGGGCg                                                            >  2:531546/1‑139 (MQ=255)                                                                            cTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGaaa                                                         >  1:584885/1‑139 (MQ=255)                                                                                         gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTaccacc                                                                                                                                       <  2:313808/48‑1 (MQ=255)                                                                                          cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaa                                           >  1:430346/1‑139 (MQ=255)                                                                                          cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaa                                           >  2:244372/1‑139 (MQ=255)                                                                                           gACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaaa                                          >  2:554376/1‑139 (MQ=255)                                                                                                            ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt                         >  1:733411/1‑139 (MQ=255)                                                                                                                  ttCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa                                                                  >  2:702997/1‑92 (MQ=255)                                                                                                                  ttCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa                                                                  <  1:702997/92‑1 (MQ=255)                                                                                                                     tttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                                >  2:303520/1‑59 (MQ=255)                                                                                                                     tttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                                <  1:303520/59‑1 (MQ=255)                                                                                                                              tCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTggg       >  1:686708/1‑139 (MQ=255)                                                                                                                               ccGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGc      >  1:230675/1‑139 (MQ=255)                                                                                                                                   accaccGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCCGTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATc  >  1:151869/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        ACAACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATC  >  NC_000913/3299866‑3300134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑