Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,353,121	C→T	*310* (TAG→TAA)	yhcC ←	putative Fe‑S oxidoreductase, Radical SAM superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,353,121	0	C	T	100.0%	164.8 / NA	51	*310* (TAG→TAA)	yhcC	putative Fe‑S oxidoreductase, Radical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/25);  total (26/25)

GGTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCG  >  NC_000913/3352986‑3353248                                                                                                                                        |                                                                                                                                ggTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTAc                                                                                                                              >  2:447558/1‑139 (MQ=255)           ataCTGCGCCAGCGGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                    <  1:436712/139‑1 (MQ=255)           ataCTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                    <  2:268159/139‑1 (MQ=255)                gcgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGc                                                                                                               <  2:663320/139‑1 (MQ=255)                 cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACATTTACGTTATAAATTGTGTGAGCAATGCGGAATTCCCCTAAACGACTTACTCCGTTGAAGGTAGcc                                                                                                              >  1:247092/1‑139 (MQ=255)                 cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  1:23089/1‑139 (MQ=255)                 cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  2:71654/1‑139 (MQ=255)                 cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  2:671106/1‑139 (MQ=255)                 cgcCAGCAGACGAATTTGC‑TCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCa                                                                                                             >  2:112399/1‑139 (MQ=255)                  gcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGATAGCCa                                                                                                             >  1:490283/1‑139 (MQ=255)                    cagcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACg                                                                                                           >  1:188655/1‑139 (MQ=255)                      gcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  2:787119/139‑1 (MQ=255)                      gcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  2:581743/139‑1 (MQ=255)                             aaTTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCa                                                                                                  <  2:320792/139‑1 (MQ=255)                                ttGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTTAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAgcg                                                                                               >  2:492976/1‑139 (MQ=255)                                ttGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAgcg                                                                                               <  1:702167/139‑1 (MQ=255)                                  gCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCATCGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:493545/139‑1 (MQ=255)                                  gCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:142190/139‑1 (MQ=255)                                          aGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTg                                                                                     <  2:279029/139‑1 (MQ=255)                                             gAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTAc                                                                                  <  1:176135/139‑1 (MQ=255)                                               aTTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                <  2:188655/139‑1 (MQ=255)                                               aTTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                <  2:21201/139‑1 (MQ=255)                                                 tCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACt                                                                                                                             <  2:242367/92‑1 (MQ=255)                                                 tCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACt                                                                                                                             >  1:242367/1‑92 (MQ=255)                                                              ccTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGAt                                                                 <  2:23089/139‑1 (MQ=255)                                                                             cccAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa                                                  <  2:187769/139‑1 (MQ=255)                                                                             cccAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa                                                  >  1:150022/1‑139 (MQ=255)                                                                                aaTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCTTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          <  1:783965/96‑1 (MQ=255)                                                                                aaTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          >  2:783965/1‑96 (MQ=255)                                                                                aaTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCa                                               >  1:515491/1‑139 (MQ=255)                                                                                     tGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTccc                                          >  2:425369/1‑139 (MQ=255)                                                                                             aCGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGGCGTCCCAGCGCCGATc                                                                                         >  1:418879/1‑84 (MQ=255)                                                                                             aCGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGGCGTCCCAGCGCCGATc                                                                                         <  2:418879/84‑1 (MQ=255)                                                                                                  ataAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGTCATGTTCATCCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGAtttt                             <  1:492976/139‑1 (MQ=255)                                                                                                   taAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTc                            >  2:527410/1‑139 (MQ=255)                                                                                                    aaaTTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCg                           <  1:425369/139‑1 (MQ=255)                                                                                                      aTTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGca                         >  1:596050/1‑139 (MQ=255)                                                                                                       ttGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcac                        <  1:86009/139‑1 (MQ=255)                                                                                                         gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         >  2:368330/1‑56 (MQ=255)                                                                                                         gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  1:368330/56‑1 (MQ=255)                                                                                                         gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             >  2:537594/1‑68 (MQ=255)                                                                                                         gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:537594/68‑1 (MQ=255)                                                                                                          tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcaccac                     >  1:779751/1‑139 (MQ=255)                                                                                                          tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcaccac                     >  2:743143/1‑139 (MQ=255)                                                                                                           gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCAc                                                                                                            >  1:744408/1‑51 (MQ=255)                                                                                                           gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCAc                                                                                                            <  2:744408/51‑1 (MQ=255)                                                                                                           gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACcaca                    >  2:125766/1‑139 (MQ=255)                                                                                                           gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACcaca                    >  1:199355/1‑139 (MQ=255)                                                                                                                    gCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGagcaa           >  1:173195/1‑139 (MQ=255)                                                                                                                          ttCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCg     >  2:789686/1‑139 (MQ=255)                                                                                                                             cccTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGTAGCGTCg  <  2:779751/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                GGTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCG  >  NC_000913/3352986‑3353248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑