Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,439,141 C→T *123* (TAG→TAA yhdN ← DUF1992 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,1410CT100.0% 138.7 / NA 43*123* (TAG→TAAyhdNDUF1992 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/18);  total (25/18)

GGCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCACTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCG  >  NC_000913/3439016‑3439276
                                                                                                                             |                                                                                                                                       
ggCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGa                                                                                                                            >  1:360767/1‑139 (MQ=255)
  cGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtt                                                                                                                          <  1:309373/139‑1 (MQ=255)
  cGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtt                                                                                                                          >  2:91845/1‑139 (MQ=255)
                 gTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTg                                                                                                           >  2:373953/1‑139 (MQ=255)
                  tACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATTCATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTg                                                                                                           <  1:566186/138‑1 (MQ=255)
                  tACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTg                                                                                                           >  2:566186/1‑138 (MQ=255)
                      cACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGc                                                                                                      >  2:408063/1‑139 (MQ=255)
                              gCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTGGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgc                                                                                              >  1:324666/1‑139 (MQ=255)
                                 ccATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgccgc                                                                                           >  2:130987/1‑139 (MQ=255)
                                          gctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAAt                                                                                  >  2:471069/1‑139 (MQ=255)
                                          gctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAAt                                                                                  >  2:564812/1‑139 (MQ=255)
                                            tgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTCTCAGCATAATCGCCGCGTAAAAAATCg                                                                                >  1:460744/1‑139 (MQ=255)
                                                              aTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGt                                                              >  2:652761/1‑139 (MQ=255)
                                                                  tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGtt                                                                                                                                    <  2:170998/65‑1 (MQ=255)
                                                                  tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGtt                                                                                                                                    >  1:170998/1‑65 (MQ=255)
                                                                  tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCa                                                          >  2:136485/1‑139 (MQ=255)
                                                                    tCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGc                                                        <  1:136485/139‑1 (MQ=255)
                                                                           ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGt                                                                                                                               >  1:417956/1‑61 (MQ=255)
                                                                           ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGt                                                                                                                               <  2:417956/61‑1 (MQ=255)
                                                                              cTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCa                                              <  1:408063/139‑1 (MQ=255)
                                                                                 ccGCCATTTTTGCCAGCTCACCAATGCGATACTTACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTa                                           >  1:476162/1‑139 (MQ=255)
                                                                                 ccGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCg                                                                                >  1:753965/1‑102 (MQ=255)
                                                                                 ccGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCg                                                                                <  2:753965/102‑1 (MQ=255)
                                                                                  cGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCcaacaa                                                                                                               <  1:55343/70‑1 (MQ=255)
                                                                                  cGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCcaacaa                                                                                                               >  2:55343/1‑70 (MQ=255)
                                                                                   gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGt                                                                                                                                     <  1:169134/47‑1 (MQ=255)
                                                                                   gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGt                                                                                                                                     >  2:169134/1‑47 (MQ=255)
                                                                                     cATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGa                                       >  1:626461/1‑139 (MQ=255)
                                                                                     cATTTTTGACAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCGTAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGa                                       <  2:476162/139‑1 (MQ=255)
                                                                                         tttGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAAt                                   <  1:652761/139‑1 (MQ=255)
                                                                                                 cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                           <  1:471069/139‑1 (MQ=255)
                                                                                                 cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                           <  2:639497/139‑1 (MQ=255)
                                                                                                 cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                           <  2:360767/139‑1 (MQ=255)
                                                                                                   cACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGGCGGCTaa                         <  1:329527/139‑1 (MQ=255)
                                                                                                   cACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                         <  2:626461/139‑1 (MQ=255)
                                                                                                   cACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                         <  2:281526/139‑1 (MQ=255)
                                                                                                           cGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGa                 <  1:91845/139‑1 (MQ=255)
                                                                                                                   acatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCgg         >  2:180170/1‑139 (MQ=255)
                                                                                                                        cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatcat    >  1:39298/1‑139 (MQ=255)
                                                                                                                          ccATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGTAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCg  >  1:801559/1‑139 (MQ=255)
                                                                                                                          ccATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCg  >  1:724364/1‑139 (MQ=255)
                                                                                                                          ccATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCg  >  1:479288/1‑139 (MQ=255)
                                                                                                                          ccATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCg  >  2:30241/1‑139 (MQ=255)
                                                                                                                             |                                                                                                                                       
GGCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCACTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCG  >  NC_000913/3439016‑3439276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: