Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,466,313	A→G	L138P (CTT→CCT)	bfr ←	bacterioferritin, iron storage and detoxification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,466,313	0	A	G	100.0%	208.9 / NA	59	L138P (CTT→CCT)	bfr	bacterioferritin, iron storage and detoxification protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/24);  total (35/24)

GCAGCATGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAAGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTCA  >  NC_000913/3466177‑3466447                                                                                                                                         |                                                                                                                                       gcagcaTGATAAACCTGGGGTATCGGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGt                                                                                                                                      >  1:542384/1‑139 (MQ=255)   agcaTGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTc                                                                                                                                    <  2:486648/139‑1 (MQ=255)        gATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAGCCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGttt                                                                                                                               >  2:527743/1‑139 (MQ=255)               cTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagcca                                                                                                                        <  1:656965/139‑1 (MQ=255)               cTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagcc                                                                                                                         >  2:128438/1‑138 (MQ=255)               cTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTccagcc                                                                                                                         <  1:128438/138‑1 (MQ=255)                 ggggTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGt                                                                                                                      >  2:143443/1‑139 (MQ=255)                  gggTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTc                                                                                                                     >  2:647536/1‑139 (MQ=255)                  gggTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTc                                                                                                                     >  2:19490/1‑139 (MQ=255)                  gggTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTc                                                                                                                     >  2:23140/1‑139 (MQ=255)                   ggTATCTGGCCTGGATCTCTTATTATACGTGGGGGTTCACCTTACCGATACGGCTCAACGTTCTTCGCGGATGTGTGCTTGCACATAATTTTGCAGGCCCATCTTCTGAATGAGATCAGGTGCCGTTTCCAGCCAGTCg                                                                                                                    <  1:361868/139‑1 (MQ=255)                   ggTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCg                                                                                                                    >  2:183611/1‑139 (MQ=255)                   ggTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCg                                                                                                                    >  1:457139/1‑139 (MQ=255)                      aTCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAGCCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGata                                                                                                                 <  1:527743/139‑1 (MQ=255)                      aTCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGata                                                                                                                 >  1:801813/1‑139 (MQ=255)                       tCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGatat                                                                                                                >  2:429642/1‑139 (MQ=255)                        cTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATg                                                                                                               >  1:12071/1‑139 (MQ=255)                        cTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATg                                                                                                               >  1:100868/1‑139 (MQ=255)                             cTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCctt                                                                                                          <  2:457139/139‑1 (MQ=255)                               ggATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCCGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCcttct                                                                                                        >  2:307211/1‑139 (MQ=255)                                       tattaACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACg                                                                                                <  2:798578/139‑1 (MQ=255)                                           aaCCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCaaa                                                                                            >  2:130425/1‑139 (MQ=255)                                                ggAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTc                                                                                       >  2:594737/1‑139 (MQ=255)                                                       tctcCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTatcatc                                                                                >  1:429006/1‑139 (MQ=255)                                                              cccGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAGAATTTCTATCATCATATcgc                                                                         >  2:356744/1‑139 (MQ=255)                                                              cccGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATcgc                                                                         >  2:619615/1‑139 (MQ=255)                                                                 gACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGc                                                                      <  1:156680/139‑1 (MQ=255)                                                                 gACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGc                                                                      <  1:594737/139‑1 (MQ=255)                                                                 gACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGc                                                                      <  2:12071/139‑1 (MQ=255)                                                                 gACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGc                                                                      <  2:100868/139‑1 (MQ=255)                                                                  acacGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCt                                                                     >  1:100361/1‑139 (MQ=255)                                                                      ggCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACg                                                                 <  1:146039/139‑1 (MQ=255)                                                                       gCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGata                                                                                                                 <  2:71895/90‑1 (MQ=255)                                                                       gCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGata                                                                                                                 >  1:71895/1‑90 (MQ=255)                                                                       gCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACg                                                                 >  1:403198/1‑138 (MQ=255)                                                                       gCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACg                                                                 <  2:403198/138‑1 (MQ=255)                                                                       gCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGt                                                                >  2:112440/1‑139 (MQ=255)                                                                       gCTCAACCTACTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCAGATCGCGGCTGACGt                                                                <  1:647536/139‑1 (MQ=255)                                                                        cTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTa                                                               <  2:401742/139‑1 (MQ=255)                                                                        cTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTa                                                               >  2:11046/1‑139 (MQ=255)                                                                         tCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTaa                                                              <  2:801813/139‑1 (MQ=255)                                                                                tcttcGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGaa                                                       >  2:108379/1‑139 (MQ=255)                                                                                      cgGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTAt                                                 <  2:367837/139‑1 (MQ=255)                                                                                           cTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCa                                            <  1:63800/139‑1 (MQ=255)                                                                                              tgCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATaa                                         >  1:206602/1‑139 (MQ=255)                                                                                                   gCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAAt                                    >  2:28434/1‑139 (MQ=255)                                                                                                     aGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTg                                  >  2:448096/1‑139 (MQ=255)                                                                                                     aGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTg                                  >  1:213305/1‑139 (MQ=255)                                                                                                        tAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCt                               >  2:444805/1‑139 (MQ=255)                                                                                                        tAATTTTGCAGGCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCt                               <  2:542384/139‑1 (MQ=255)                                                                                                                   gCCCATCTTCTGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAAttc                    >  2:349443/1‑139 (MQ=255)                                                                                                                         cttctGAATCAGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTcgcg              <  1:632701/139‑1 (MQ=255)                                                                                                                                   aGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGaa                                                       <  2:459214/88‑1 (MQ=255)                                                                                                                                   aGATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGaa                                                       >  1:459214/1‑88 (MQ=255)                                                                                                                                    gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTa                                                                                     <  2:587800/57‑1 (MQ=255)                                                                                                                                    gATCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTa                                                                                     >  1:587800/1‑57 (MQ=255)                                                                                                                                     aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGTGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTCa  >  2:20290/1‑139 (MQ=255)                                                                                                                                     aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTCa  >  2:801174/1‑139 (MQ=255)                                                                                                                                     aTCAGGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTCa  <  2:94502/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GCAGCATGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAACCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTCTGAATCAGATCAAGTTCCGTTTCCAGCCAGTCGATATGGCCTTCTTCATCACGCAAAATTTCTATCATCATATCGCGGCTGACGTAATCATGAACGCTATCGGCATAACCAATTGCCTCACGCAAATTCTTCGCGCCATCCAGCTCA  >  NC_000913/3466177‑3466447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑