Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,942	A→G	Y217H (TAC→CAC)	php ←	phosphotriesterase homology protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,942	0	A	G	100.0%	120.3 / NA	34	Y217H (TAC→CAC)	php	phosphotriesterase homology protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

AGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCG  >  NC_000913/3507806‑3508066                                                                                                                                         |                                                                                                                             aGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGggtggt                                                                                                                            >  2:704529/1‑139 (MQ=255)   tAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTaa                                                                                                                          <  1:401306/139‑1 (MQ=255)        aGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAAACCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGATAACTGtt                                                                                                                     >  1:485695/1‑139 (MQ=255)            aTAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTg                                                                                                                 >  2:485965/1‑139 (MQ=255)            aTAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTg                                                                                                                 >  1:785163/1‑139 (MQ=255)                     accaccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGgtg                                                                                                        <  1:735190/139‑1 (MQ=255)                             tGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTg                                                                                                >  2:197782/1‑139 (MQ=255)                              ggCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGc                                                                                               >  1:650006/1‑139 (MQ=255)                                cTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCAc                                                                                             >  2:710708/1‑139 (MQ=255)                                   ttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                          <  1:743291/139‑1 (MQ=255)                                        tGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACgcgc                                                                                     <  1:199885/139‑1 (MQ=255)                                         gggAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGcc                                                                                    <  1:485965/139‑1 (MQ=255)                                                gcgcGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcg                                                                             <  2:252253/139‑1 (MQ=255)                                                               cGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAtg                                                              >  1:274525/1‑139 (MQ=255)                                                                  cAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAtgttg                                                           >  2:548394/1‑139 (MQ=255)                                                                    gCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTc                                                         >  1:29156/1‑139 (MQ=255)                                                                    gCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCAAGATCGATCATCTTCAAAATGTTGTc                                                         >  1:509293/1‑139 (MQ=255)                                                                     cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCg                                                        >  2:159738/1‑139 (MQ=255)                                                                             ggTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTc                                                <  1:285998/139‑1 (MQ=255)                                                                                   gCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                               >  1:545226/1‑118 (MQ=255)                                                                                   gCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                               <  2:545226/118‑1 (MQ=255)                                                                                         cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                    >  2:6947/1‑139 (MQ=255)                                                                                                 cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                          <  1:758201/93‑1 (MQ=255)                                                                                                 cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                          >  2:758201/1‑93 (MQ=255)                                                                                                  gTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                          >  2:597042/1‑92 (MQ=255)                                                                                                  gTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                          <  1:597042/92‑1 (MQ=255)                                                                                                  gTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAAc                           >  1:697144/1‑139 (MQ=255)                                                                                                         aTGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcg                    <  1:6947/139‑1 (MQ=255)                                                                                                         aTGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTCTTCTTGCCGATGGTGTCGAACTGCACCTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGCGACCAACGGTGAcg                    <  2:485695/139‑1 (MQ=255)                                                                                                              gCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa               <  2:195905/139‑1 (MQ=255)                                                                                                                      aTACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATc         <  2:266105/137‑1 (MQ=255)                                                                                                                      aTACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATc         >  1:266105/1‑137 (MQ=255)                                                                                                                           cTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGAACGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCg  <  2:493191/139‑1 (MQ=255)                                                                                                                                 gTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                          >  2:495940/1‑45 (MQ=255)                                                                                                                                 gTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                          <  1:495940/45‑1 (MQ=255)                                                                                                                                         |                                                                                                                             AGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCG  >  NC_000913/3507806‑3508066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑