Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,732,319	G→A	G40E (GGG→GAG)	xylG →	D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,732,319	0	G	A	100.0%	224.5 / NA	68	G40E (GGG→GAG)	xylG	D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/30);  total (38/30)

TTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTAT  >  NC_000913/3732186‑3732448                                                                                                                                      |                                                                                                                                  ttAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTg                                                                                                                              <  2:179342/139‑1 (MQ=255)  tAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTgg                                                                                                                             <  1:334058/139‑1 (MQ=255)    ggTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTa                                                                                                                           >  2:381491/1‑139 (MQ=255)         aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                      >  2:280333/1‑139 (MQ=255)         aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                      >  2:72664/1‑139 (MQ=255)         aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                      >  2:734152/1‑139 (MQ=255)             gTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGc                                                                                                                  <  1:280333/139‑1 (MQ=255)              ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                 >  2:165912/1‑139 (MQ=255)              ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                 <  1:481794/139‑1 (MQ=255)                aTGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtga                                                                                                               >  1:735932/1‑139 (MQ=255)                  gCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtgatg                                                                                                             >  2:301264/1‑139 (MQ=255)                       aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGt                                                                                                        >  1:6301/1‑139 (MQ=255)                        tCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                       <  1:734152/139‑1 (MQ=255)                         cTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGc                                                                                                      <  2:754271/139‑1 (MQ=255)                          tACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGGAAGTGCt                                                                                                     <  2:557097/139‑1 (MQ=255)                          tACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     <  1:10210/139‑1 (MQ=255)                             ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                  >  2:58282/1‑139 (MQ=255)                             ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                  >  2:760537/1‑139 (MQ=255)                             ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                  <  2:330755/139‑1 (MQ=255)                               gAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgtg                                                                                                <  1:290314/139‑1 (MQ=255)                               gAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgtg                                                                                                >  1:404298/1‑139 (MQ=255)                                 aaTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGt                                                                                              <  1:10464/139‑1 (MQ=255)                                  aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTa                                                                                             >  1:606213/1‑139 (MQ=255)                                  aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTa                                                                                             >  2:203457/1‑139 (MQ=255)                                      agaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     >  2:217330/1‑127 (MQ=255)                                      agaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     <  1:217330/127‑1 (MQ=255)                                            ttACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCa                                                                                   <  1:58282/139‑1 (MQ=255)                                             tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                 >  2:409683/1‑108 (MQ=255)                                             tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                 <  1:409683/108‑1 (MQ=255)                                             tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtg                                                                                                    <  2:376443/121‑1 (MQ=255)                                             tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtg                                                                                                    >  1:376443/1‑121 (MQ=255)                                             tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCAt                                                                                  >  2:153333/1‑139 (MQ=255)                                               ccAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                       <  2:93145/116‑1 (MQ=255)                                               ccAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                       >  1:93145/1‑116 (MQ=255)                                                  aaaCCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACg                                                                                                                   <  1:390186/101‑1 (MQ=255)                                                  aaaCCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACg                                                                                                                   >  2:390186/1‑101 (MQ=255)                                                    aCCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCt                                                                           >  2:741927/1‑139 (MQ=255)                                                       ttCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtga                                                                                                               >  1:302654/1‑100 (MQ=255)                                                       ttCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtga                                                                                                               <  2:302654/100‑1 (MQ=255)                                                               tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGaa                                                                >  2:702603/1‑139 (MQ=255)                                                               tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGaa                                                                >  1:477126/1‑139 (MQ=255)                                                                    aGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAattat                                                           >  2:139292/1‑139 (MQ=255)                                                                      gCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                       <  2:512805/93‑1 (MQ=255)                                                                      gCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                       >  1:512805/1‑93 (MQ=255)                                                                         aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     >  1:705003/1‑92 (MQ=255)                                                                         aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     <  2:705003/92‑1 (MQ=255)                                                                         aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                      <  1:381491/139‑1 (MQ=255)                                                                         aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                      >  1:697210/1‑139 (MQ=255)                                                                         aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                      >  2:723139/1‑139 (MQ=255)                                                                         aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                      >  1:570088/1‑139 (MQ=255)                                                                           tGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCg                                                    >  2:217245/1‑139 (MQ=255)                                                                           tGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCg                                                    >  1:764632/1‑139 (MQ=255)                                                                             aTAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCggg                                                  <  1:165912/139‑1 (MQ=255)                                                                                  gTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGagaag                                             <  1:625519/139‑1 (MQ=255)                                                                                     tGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAgaga                                          >  2:791037/1‑139 (MQ=255)                                                                                      gCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGAt                                         >  1:647859/1‑139 (MQ=255)                                                                                       cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACg                                                                                                                   >  2:68632/1‑64 (MQ=255)                                                                                       cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACg                                                                                                                   <  1:68632/64‑1 (MQ=255)                                                                                       cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                 <  1:750709/82‑1 (MQ=255)                                                                                       cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                 >  2:750709/1‑82 (MQ=255)                                                                                       cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGAtt                                        >  2:126551/1‑139 (MQ=255)                                                                                           cGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAgg                                    <  1:791037/139‑1 (MQ=255)                                                                                                 aaTGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTc                              >  2:253650/1‑139 (MQ=255)                                                                                                        gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCcg                       <  2:570088/139‑1 (MQ=255)                                                                                                               cGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATAcc                <  1:139292/139‑1 (MQ=255)                                                                                                               cGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATAcc                <  2:404298/139‑1 (MQ=255)                                                                                                                     aCTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGCGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGc          >  1:505559/1‑139 (MQ=255)                                                                                                                             gggAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTAt  <  1:702603/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  TTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTAT  >  NC_000913/3732186‑3732448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑