Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,047	C→T	A316V (GCT→GTT)  *345* (TAG→TAA)	yibQ → waaH ←	putative polysaccharide deacetylase LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,047	0	C	T	100.0%	132.9 / NA	41	A316V (GCT→GTT)  *345* (TAG→TAA)	yibQ waaH	putative polysaccharide deacetylase LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/23);  total (18/23)

CCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGG  >  NC_000913/3788914‑3789176                                                                                                                                      |                                                                                                                                  ccGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCTAAAGCATCAGCCAGAGCTCGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTTgcg                                                                                                                              <  1:514893/139‑1 (MQ=255)          ccTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTaaa                                                                                                                     <  1:637523/139‑1 (MQ=255)              tttATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcg                                                                                                                 >  2:554710/1‑139 (MQ=255)                        tCGCTTCTTTGAAGTGTTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTaa                                                                                                       >  2:444381/1‑139 (MQ=255)                        tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcg                                                                                                                 <  1:784648/129‑1 (MQ=255)                        tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcg                                                                                                                 >  2:784648/1‑129 (MQ=255)                        tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcgcg                                                                                                          <  1:703895/136‑1 (MQ=255)                        tCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcgcg                                                                                                          >  2:703895/1‑136 (MQ=255)                                    aGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCg                                                                                           <  2:710270/139‑1 (MQ=255)                                     gTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGa                                                                                          <  2:653295/139‑1 (MQ=255)                                        ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                       >  2:392534/1‑139 (MQ=255)                                        ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                       >  1:558394/1‑139 (MQ=255)                                                    aTCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTcgcg                                                                           <  2:410394/139‑1 (MQ=255)                                                               cACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATa                                                                <  1:59208/139‑1 (MQ=255)                                                                       tCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGaaa                                                        >  1:153192/1‑139 (MQ=255)                                                                          ttttCTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                     <  1:199217/139‑1 (MQ=255)                                                                          tttACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                     <  1:585447/139‑1 (MQ=255)                                                                           ttACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAcc                                                    <  1:554710/139‑1 (MQ=255)                                                                           ttACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAcc                                                    <  1:444381/139‑1 (MQ=255)                                                                             aCTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACcac                                                  >  2:108683/1‑139 (MQ=255)                                                                                tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcgc                                                                                                                <  2:123688/74‑1 (MQ=255)                                                                                tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcgc                                                                                                                >  1:123688/1‑74 (MQ=255)                                                                                tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGt                                               <  1:218810/139‑1 (MQ=255)                                                                                  cAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCACATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTaa                                             >  1:432212/1‑139 (MQ=255)                                                                                     cATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCt                                                                                                               <  2:167402/70‑1 (MQ=255)                                                                                     cATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCt                                                                                                               >  1:167402/1‑70 (MQ=255)                                                                                         aGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTgg                                      >  1:337409/1‑139 (MQ=255)                                                                                                aGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGa                               >  2:42868/1‑139 (MQ=255)                                                                                                 ggCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGAc                              >  1:343672/1‑139 (MQ=255)                                                                                                     ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAg                                                >  2:332649/1‑117 (MQ=255)                                                                                                     ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAg                                                <  1:332649/117‑1 (MQ=255)                                                                                                     ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTc                          >  1:657121/1‑139 (MQ=255)                                                                                                            aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTgc                   >  2:421283/1‑139 (MQ=255)                                                                                                            aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTgc                   >  2:52135/1‑139 (MQ=255)                                                                                                               gCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGca                <  2:657121/139‑1 (MQ=255)                                                                                                                 ccGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGcaca              <  2:343672/139‑1 (MQ=255)                                                                                                                  cGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACAt             <  2:558394/139‑1 (MQ=255)                                                                                                                           gAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCa    <  2:50144/139‑1 (MQ=255)                                                                                                                           gAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCa    <  1:726441/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg  <  1:108683/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg  <  2:557168/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  CCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGG  >  NC_000913/3788914‑3789176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑