Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,818,584	G→A	*275* (TAG→TAA)	dinD →	DNA damage‑inducible protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,818,584	0	G	A	100.0%	106.0 / NA	34	*275* (TAG→TAA)	dinD	DNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/18);  total (16/18)

CACACTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTATGCATT  >  NC_000913/3818451‑3818717                                                                                                                                      |                                                                                                                                      cacaaTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGaa                                                                                                                                  >  2:346008/1‑139 (MQ=255)      tttGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaa                                                                                                                             >  2:375819/1‑139 (MQ=255)       ttGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaa                                                                                                                            <  1:346008/139‑1 (MQ=255)                gTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCtttt                                                                                                                   >  2:257586/1‑139 (MQ=255)                gTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCtttt                                                                                                                   <  1:281462/139‑1 (MQ=255)                  cGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTttg                                                                                                                 >  2:243707/1‑139 (MQ=255)                     aaGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTttgttg                                                                                                              <  2:287339/139‑1 (MQ=255)                          gAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGttgattga                                                                                                         <  2:632260/139‑1 (MQ=255)                           aGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaaa                                                                                                                           >  2:4602/1‑120 (MQ=255)                           aGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaaa                                                                                                                           <  1:4602/120‑1 (MQ=255)                                       cAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCg                                                                                            >  2:757798/1‑139 (MQ=255)                                        aGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGt                                                                                           <  1:757798/139‑1 (MQ=255)                                              ttGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCa                                                                                     <  1:784349/139‑1 (MQ=255)                                                ggCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTttg                                                                                                                 <  1:473825/109‑1 (MQ=255)                                                ggCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTttg                                                                                                                 >  2:473825/1‑109 (MQ=255)                                                      aCCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAg                                                                             <  1:375819/139‑1 (MQ=255)                                                        cATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                           <  2:224264/139‑1 (MQ=255)                                                          tGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTAGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGc                                                                         >  2:527076/1‑139 (MQ=255)                                                          tGCCTGAGGAGTTGCCAACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGc                                                                         <  2:171028/139‑1 (MQ=255)                                                           gCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                                        <  2:569758/139‑1 (MQ=255)                                                                aggagTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGaa                                                                   <  2:34148/139‑1 (MQ=255)                                                                 ggagTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAac                                                                  >  1:265880/1‑139 (MQ=255)                                                                    gTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAAcaca                                                               <  1:257586/139‑1 (MQ=255)                                                                               cGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAata                                                    >  2:782977/1‑139 (MQ=255)                                                                                  aGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTg                                                 <  1:104305/139‑1 (MQ=255)                                                                                                    tGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCt                               <  2:656464/139‑1 (MQ=255)                                                                                                             gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTaa                      >  1:642006/1‑139 (MQ=255)                                                                                                              tgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAAc                     <  2:265880/139‑1 (MQ=255)                                                                                                               gtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                                        <  2:361994/87‑1 (MQ=255)                                                                                                               gtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                                        >  1:361994/1‑87 (MQ=255)                                                                                                                tAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCt                   >  1:123595/1‑139 (MQ=255)                                                                                                                        aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTc           >  1:222643/1‑139 (MQ=255)                                                                                                                        aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTc           >  1:118459/1‑139 (MQ=255)                                                                                                                                 aaaTAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTATGCAtt  >  1:113648/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                      CACACTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTATGCATT  >  NC_000913/3818451‑3818717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑