Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,075,454	G→A	*262* (TAG→TAA)	yihW →	putative transcriptional regulator for sulphoquinovose utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,075,454	0	G	A	100.0%	112.7 / NA	36	*262* (TAG→TAA)	yihW	putative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTG  >  NC_000913/4075318‑4075586                                                                                                                                         |                                                                                                                                     aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                                    >  1:352720/1‑139 (MQ=255) aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAAGCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                                    >  2:203617/1‑139 (MQ=255)     ttCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAttttt                                                                                                                                >  2:420330/1‑139 (MQ=255)         gcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGa                                                                                                                            >  2:406340/1‑139 (MQ=255)           ggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATGCTTCCCCAGGAAGACGCGTAAATTTTTGTGAcc                                                                                                                          <  2:88735/139‑1 (MQ=255)               gTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTa                                                                                                                      >  1:718226/1‑139 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                   <  1:142047/139‑1 (MQ=255)                   aaaTTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCt                                                                                                                  <  1:460428/139‑1 (MQ=255)                             cGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATAc                                                                                                        >  1:141541/1‑139 (MQ=255)                                 gCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTg                                                                                                    >  1:419436/1‑139 (MQ=255)                                  cacaGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGc                                                                                                   >  1:700885/1‑139 (MQ=255)                                          aCTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGc                                                                                           <  1:198331/139‑1 (MQ=255)                                          aCTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGc                                                                                           >  1:510610/1‑139 (MQ=255)                                            tGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCa                                                                                         >  1:236789/1‑139 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGc                                                                        >  1:216540/1‑139 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGc                                                                        >  2:310266/1‑139 (MQ=255)                                                               cTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                                      <  2:108989/139‑1 (MQ=255)                                                               cTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                                      >  2:411718/1‑139 (MQ=255)                                                                            cgcTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCtt                                                         >  2:65974/1‑139 (MQ=255)                                                                            cgcTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTCTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCtt                                                         >  1:681404/1‑139 (MQ=255)                                                                                  aaTCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGgcg                                                   <  2:659911/139‑1 (MQ=255)                                                                                                   aGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAAt                                  <  2:141541/139‑1 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                   >  1:295804/1‑53 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                   <  2:295804/53‑1 (MQ=255)                                                                                                              aaTCATTCTTCCGCAGGAAGGCCCGTTAATTTTTGTGACCGTTAATCTGTCTTCTTACCTTGCCACATAGCCAAACCCCTCCTTTCGCGCTACAGTTAATTTCTTGTGTCGCGAAAGGAGGCAAAAATGCTCTATATCt                       <  1:411718/139‑1 (MQ=255)                                                                                                              aaTCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt                       <  2:442214/139‑1 (MQ=255)                                                                                                              aaTCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt                       <  1:406340/139‑1 (MQ=255)                                                                                                                tCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCttt                     <  1:310266/139‑1 (MQ=255)                                                                                                                    tcttcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGAtt                 >  1:167344/1‑139 (MQ=255)                                                                                                                            aGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAAt         <  2:721505/139‑1 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGAtt   >  2:365062/1‑139 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGAGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGAtt   >  2:476807/1‑139 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCca                                                                                                 >  2:635165/1‑44 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCca                                                                                                 <  1:635165/44‑1 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt                       <  2:432927/118‑1 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt                       >  1:432927/1‑118 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTg  >  2:31527/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTG  >  NC_000913/4075318‑4075586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑