Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,849	A→G	intergenic (+21/‑131)	ghxP → / → yjcE	guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,849	0	A	G	100.0%	163.7 / NA	46	intergenic (+21/‑131)	ghxP/yjcE	guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)

GTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGG  >  NC_000913/4279734‑4279983                                                                                                                    |                                                                                                                                       gtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGttt                                                                                                                 >  2:759486/1‑139 (MQ=255)    tGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTa                                                                                                              >  1:17622/1‑139 (MQ=255)        ttCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAtttt                                                                                                          >  1:613176/1‑139 (MQ=255)         tCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtc                                                                                                         <  1:397532/139‑1 (MQ=255)                 aaggCGCAAGTTGAACATCCGTAAGGTCGTTATCGCCGGGGCGCTGGTTAACTTTTTTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                                 <  1:572338/137‑1 (MQ=255)                 aTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                                 <  1:622958/139‑1 (MQ=255)                 aTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCCATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                                 <  2:424718/139‑1 (MQ=255)                          gTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAg                                                                                        <  1:192035/139‑1 (MQ=255)                                  tCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGGTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTTGCCGTTTTTATTTTCTCTGCACATTGTGTGAGCTTTTGCg                                                                                <  1:263439/139‑1 (MQ=255)                                     gTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg                                                                                                                                     >  2:401257/1‑83 (MQ=255)                                     gTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg                                                                                                                                     <  1:401257/83‑1 (MQ=255)                                              ttATCGCCGTGGCGCTGGTGACCTTCTATGTGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaaaa                                                                    >  2:757394/1‑139 (MQ=255)                                              ttATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                                 >  1:343110/1‑110 (MQ=255)                                              ttATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                                 <  2:343110/110‑1 (MQ=255)                                                     cGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATgag                                                             <  1:134025/139‑1 (MQ=255)                                                       tGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt                                                                                                             >  1:185531/1‑89 (MQ=255)                                                       tGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt                                                                                                             <  2:185531/89‑1 (MQ=255)                                                         gcgcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCGTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAtt                                                         <  1:584778/139‑1 (MQ=255)                                                                         aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtc                                                                                                         >  1:447963/1‑75 (MQ=255)                                                                         aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtc                                                                                                         <  2:447963/75‑1 (MQ=255)                                                                          tGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTtc                                        >  2:789943/1‑139 (MQ=255)                                                                              ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCa                                                   >  2:345794/1‑124 (MQ=255)                                                                              ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCa                                                   <  1:345794/124‑1 (MQ=255)                                                                              ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAg                                    >  2:312342/1‑139 (MQ=255)                                                                              ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAg                                    >  2:157350/1‑139 (MQ=255)                                                                               gTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                                   <  1:312342/139‑1 (MQ=255)                                                                               gTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                                   >  1:719533/1‑139 (MQ=255)                                                                               gTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                                   <  1:757394/139‑1 (MQ=255)                                                                               gTGGCTGGGCTATCTAATCTTGCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAGTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAAACAGGCATAACGTCTTTCTCAGa                                   <  1:561095/139‑1 (MQ=255)                                                                                 ggCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCg                                                                                >  1:27833/1‑92 (MQ=255)                                                                                 ggCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCg                                                                                <  2:27833/92‑1 (MQ=255)                                                                                 ggCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATc                                 >  1:389274/1‑139 (MQ=255)                                                                                  gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACg                                              >  2:224386/1‑125 (MQ=255)                                                                                  gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACg                                              <  1:224386/125‑1 (MQ=255)                                                                                    tGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCg                                                                                                     >  2:634080/1‑68 (MQ=255)                                                                                    tGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCg                                                                                                     <  1:634080/68‑1 (MQ=255)                                                                                       gCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaa                           >  1:24109/1‑139 (MQ=255)                                                                                        cTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaaa                          >  2:754061/1‑139 (MQ=255)                                                                                            cTAATCTTTCATCGGATTCTGAAAGCGTGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACTTTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACAt                      <  1:746826/139‑1 (MQ=255)                                                                                                     cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgt                                                                                           >  2:165619/1‑61 (MQ=255)                                                                                                     cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgt                                                                                           <  1:165619/61‑1 (MQ=255)                                                                                                     cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACAt                      >  1:782483/1‑130 (MQ=255)                                                                                                     cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACAt                      <  2:782483/130‑1 (MQ=255)                                                                                                     cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAg             >  2:106505/1‑139 (MQ=255)                                                                                                       tCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAggg           >  1:793281/1‑139 (MQ=255)                                                                                                                gAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATgg  <  2:17622/139‑1 (MQ=255)                                                                                                                    |                                                                                                                                       GTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGG  >  NC_000913/4279734‑4279983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑