Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,240	A→G	I280T (ATC→ACC)	mdtO ←	membrane translocase (MDR) of MdtNOP efflux pump, PET family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,240	0	A	G	100.0%	127.8 / NA	36	I280T (ATC→ACC)	mdtO	membrane translocase (MDR) of MdtNOP efflux pump, PET family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

CGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGT  >  NC_000913/4302125‑4302373                                                                                                                    |                                                                                                                                      cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                                <  2:672176/139‑1 (MQ=255) cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                                >  2:280824/1‑139 (MQ=255)  gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTTACTGATCCGCCAGTCGCTTTGCCAGCTCTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGATTCTGAAGCTAATTTTTGTCGGa                                                                                                               >  1:622291/1‑139 (MQ=255)  gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGa                                                                                                               >  1:339649/1‑139 (MQ=255)   tCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGaa                                                                                                              <  1:424144/139‑1 (MQ=255)                     ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                            >  1:531397/1‑139 (MQ=255)                     ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                            <  2:225630/139‑1 (MQ=255)                      gTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGaa                                                                                           >  2:636808/1‑139 (MQ=255)                       ttACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAAt                                                                                          <  2:622291/139‑1 (MQ=255)                              cccGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCaaa                                                                                   <  2:339649/139‑1 (MQ=255)                                cGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                                 <  1:443302/107‑1 (MQ=255)                                cGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                                 >  2:443302/1‑107 (MQ=255)                                 gTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAaga                                                                                <  2:639170/139‑1 (MQ=255)                                  tGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAagag                                                                               >  1:358806/1‑139 (MQ=255)                                    ccgccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGt                                                                             >  1:780662/1‑139 (MQ=255)                                       ccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTggg                                                                          <  1:776402/139‑1 (MQ=255)                                              ttcactttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAg                                                                   <  1:158509/139‑1 (MQ=255)                                                   tttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGAtt                                                              <  1:21806/139‑1 (MQ=255)                                                         tGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                                        <  1:712852/139‑1 (MQ=255)                                                         tGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                                        <  2:358806/139‑1 (MQ=255)                                                          gATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTc                                                       >  2:218803/1‑139 (MQ=255)                                                           aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                                 >  1:500408/1‑80 (MQ=255)                                                           aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                                 <  2:500408/80‑1 (MQ=255)                                                           aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCg                                                      >  1:435654/1‑139 (MQ=255)                                                            tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                                     >  1:219208/1‑139 (MQ=255)                                                              cGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                   <  1:636808/139‑1 (MQ=255)                                                              cGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                   <  2:583172/139‑1 (MQ=255)                                                              cGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                   <  1:280824/139‑1 (MQ=255)                                                                     cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTa                                            >  2:92592/1‑139 (MQ=255)                                                                                    gtccTTCAGCAACTGCATGCTGCTGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATACTTGCCTGACAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCAc                             <  1:622833/137‑1 (MQ=255)                                                                                    gACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCAc                             <  2:435654/139‑1 (MQ=255)                                                                                     aCCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                         <  1:503721/110‑1 (MQ=255)                                                                                     aCCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                         >  2:503721/1‑110 (MQ=255)                                                                                                cGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTTCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCTTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGcca                 >  1:75010/1‑139 (MQ=255)                                                                                                            gCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTg     <  1:369825/139‑1 (MQ=255)                                                                                                               tgttgGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGt  >  2:263833/1‑139 (MQ=255)                                                                                                                    |                                                                                                                                      CGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGT  >  NC_000913/4302125‑4302373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑