Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,336,405 A→G S209S (AGT→AGC)  adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,336,4050AG94.1% 107.8 / ‑2.1 34S209S (AGT→AGC) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (16/16);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

GCGGCATTAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCC  >  NC_000913/4336271‑4336527
                                                                                                                                      |                                                                                                                          
gCGGCATTAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCttt                                                                                                                        <  2:220760/139‑1 (MQ=255)
   gCATTAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCca                                                                                                                     <  1:352755/139‑1 (MQ=255)
         gggATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                               >  1:782450/1‑139 (MQ=255)
         gggATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGa                                                                                                               >  2:191683/1‑139 (MQ=255)
              catcCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                               >  1:176973/1‑134 (MQ=255)
              catcCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                               <  2:176973/134‑1 (MQ=255)
                           gcgGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTaa                                                                                             >  1:451236/1‑139 (MQ=255)
                             ggtggtAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACg                                                                                           >  2:39234/1‑139 (MQ=255)
                                   aGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                     <  2:556433/139‑1 (MQ=255)
                                         tACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACtt                                                                               >  1:786395/1‑139 (MQ=255)
                                           cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                               <  1:360269/121‑1 (MQ=255)
                                           cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                               >  2:360269/1‑121 (MQ=255)
                                           cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTg                                                                             <  2:782450/139‑1 (MQ=255)
                                              aGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAAt                                                                          <  1:191683/139‑1 (MQ=255)
                                                        ggcAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAg                                                                <  2:786395/139‑1 (MQ=255)
                                                          caatcaatACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                               <  1:770261/106‑1 (MQ=255)
                                                          caatcaatACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                               >  2:770261/1‑106 (MQ=255)
                                                          caatcaatACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                     >  2:785214/1‑116 (MQ=255)
                                                          caatcaatACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                     <  1:785214/116‑1 (MQ=255)
                                                             tcaatACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGcc                                                           <  2:178272/139‑1 (MQ=255)
                                                                 tACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                     >  1:599830/1‑109 (MQ=255)
                                                                 tACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                     <  2:290605/109‑1 (MQ=255)
                                                                 tACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                     <  2:599830/109‑1 (MQ=255)
                                                                 tACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                     >  1:290605/1‑109 (MQ=255)
                                                                              gtggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTcc                                          >  2:271499/1‑139 (MQ=255)
                                                                               tggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCa                                         <  1:449086/139‑1 (MQ=255)
                                                                                  cGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgc                                      >  2:678210/1‑139 (MQ=255)
                                                                                         gACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCata                               <  1:271499/139‑1 (MQ=255)
                                                                                             ttGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAgg                                                       <  2:207677/111‑1 (MQ=255)
                                                                                             ttGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAgg                                                       >  1:207677/1‑111 (MQ=255)
                                                                                              tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGt                          >  1:710293/1‑139 (MQ=255)
                                                                                                   ttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcac                     >  2:302200/1‑139 (MQ=255)
                                                                                                      gggTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcaccac                  <  2:380539/139‑1 (MQ=255)
                                                                                                                      cTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGcc  >  1:421839/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                          
GCGGCATTAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCC  >  NC_000913/4336271‑4336527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑