Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,377,189 C→T H105H (CAC→CAT
*178* (TAG→TAA
sugE →
blc ←
multidrug efflux system protein
outer membrane lipoprotein cell division and growth lipocalin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,377,1890CT100.0% 83.1 / NA 27H105H (CAC→CAT
*178* (TAG→TAA
sugE
blc
multidrug efflux system protein
outer membrane lipoprotein cell division and growth lipocalin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/14);  total (13/14)

CGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGC  >  NC_000913/4377054‑4377319
                                                                                                                                       |                                                                                                                                  
cGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCattaa                                                                                                                                 <  2:287763/139‑1 (MQ=255)
      gACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCa                                                                                                                           <  2:552555/139‑1 (MQ=255)
        cGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAgg                                                                                                                         <  1:348930/139‑1 (MQ=255)
            tATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctgc                                                                                                                     >  1:106720/1‑139 (MQ=255)
            tATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGgctgc                                                                                                                     >  1:254165/1‑139 (MQ=255)
                      gTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCaaa                                                                                                           <  2:276736/139‑1 (MQ=255)
                                 cATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTg                                                                                                <  2:254165/139‑1 (MQ=255)
                                               tgctgcTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTc                                                                                  >  1:795950/1‑139 (MQ=255)
                                                         tGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTcgcg                                                                        >  2:756376/1‑139 (MQ=255)
                                                            gTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACt                                                                     <  1:676266/139‑1 (MQ=255)
                                                                    aCCCGTTGCGCCTGGCGAGGCAGGCGTTAATCGTATTGGGTACTATTAGTCTGAAACTCAGCATTCGTTAACTACCAGGCTGCTGTACCCAAATCAATTTCCTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGcct                                                             <  1:582233/139‑3 (MQ=255)
                                                                         aTGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCt                                                        >  2:448836/1‑139 (MQ=255)
                                                                                tGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCAc                                                 <  2:664111/139‑1 (MQ=255)
                                                                                    gAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTcc                                                                                 >  1:360611/1‑103 (MQ=255)
                                                                                    gAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTcc                                                                                 <  2:360611/103‑1 (MQ=255)
                                                                                         tGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCattaacta                                                                                                                              >  1:440138/1‑53 (MQ=255)
                                                                                         tGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCattaacta                                                                                                                              <  2:440138/53‑1 (MQ=255)
                                                                                                   cGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGc                              <  2:678507/139‑1 (MQ=255)
                                                                                                            gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAgg                                                                                                                         >  1:147649/1‑39 (MQ=255)
                                                                                                            gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAgg                                                                                                                         <  2:147649/39‑1 (MQ=255)
                                                                                                            gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGgag                     <  2:459617/139‑1 (MQ=255)
                                                                                                            gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGgag                     <  1:427905/139‑1 (MQ=255)
                                                                                                                   ggTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATcc              <  2:670389/139‑1 (MQ=255)
                                                                                                                    gTCTGAAACTCAGCGCTCATTAACTACCAGGCTGCTGTACACAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCca             >  2:487444/1‑139 (MQ=255)
                                                                                                                    gTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTa                                                                                                                 <  2:437419/39‑1 (MQ=255)
                                                                                                                    gTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTa                                                                                                                 >  1:437419/1‑39 (MQ=255)
                                                                                                                    gTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATTAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCca             >  1:752713/1‑139 (MQ=255)
                                                                                                                       tGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAg          >  1:45101/1‑139 (MQ=255)
                                                                                                                              cAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTcg   >  2:793780/1‑139 (MQ=255)
                                                                                                                               aGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTACACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTcgc  >  2:507351/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                  
CGTGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGC  >  NC_000913/4377054‑4377319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: