| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,422,793 | C→T | A203V (GCC→GTC) | ulaD → | 3‑keto‑L‑gulonate 6‑phosphate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,422,793 | 0 | C | T | 100.0% | 85.9 / NA | 28 | A203V (GCC→GTC) | ulaD | 3‑keto‑L‑gulonate 6‑phosphate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/15); total (13/15) | |||||||||||
TTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGA > NC_000913/4422662‑4422913 | ttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGt < 1:499566/139‑1 (MQ=255) tCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTc > 2:231779/1‑139 (MQ=255) ggCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAAc > 1:663159/1‑139 (MQ=255) tCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc < 2:663159/139‑1 (MQ=255) tcaccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGc > 2:158543/1‑139 (MQ=255) caccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCt < 1:158543/139‑1 (MQ=255) accgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTg < 1:540713/139‑1 (MQ=255) caccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg < 1:269581/139‑1 (MQ=255) cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg < 1:327519/139‑1 (MQ=255) cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg < 2:658903/139‑1 (MQ=255) ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa < 1:281909/110‑1 (MQ=255) ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa > 2:281909/1‑110 (MQ=255) aGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtg < 2:52609/139‑1 (MQ=255) gTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCcaaacaa > 1:547879/1‑139 (MQ=255) ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCAc < 2:92623/66‑1 (MQ=255) ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCAc > 1:92623/1‑66 (MQ=255) ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg > 2:224442/1‑139 (MQ=255) ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg > 2:442159/1‑139 (MQ=255) ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg > 2:554513/1‑139 (MQ=255) ttCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCcaa > 2:157472/1‑118 (MQ=255) ttCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCcaa < 1:157472/118‑1 (MQ=255) cGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTgggg > 2:416424/1‑90 (MQ=255) cGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTgggg < 1:416424/90‑1 (MQ=255) tagtaTCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCggg < 1:554513/139‑1 (MQ=255) gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTg > 2:237652/1‑131 (MQ=255) gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTg < 1:237652/131‑1 (MQ=255) ccGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa < 1:442159/139‑1 (MQ=255) ccGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa > 2:735303/1‑139 (MQ=255) | TTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGA > NC_000913/4422662‑4422913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |