Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,577,958 C→T M1M (GTG→ATG) †
*460* (TAG→TAA
mcrC ←
mcrB ←
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,577,9580CT100.0% 235.8 / NA 72M1M (GTG→ATG) †
*460* (TAG→TAA
mcrC
mcrB
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/28);  total (44/28)

CATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577826‑4578091
                                                                                                                                    |                                                                                                                                     
catacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  2:506896/139‑1 (MQ=255)
 atacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGt                                                                                                                                >  1:21814/1‑139 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  2:300998/136‑1 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  1:300998/1‑136 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  2:72753/1‑139 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  2:346651/1‑139 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  1:154705/1‑139 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  1:726181/1‑139 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  1:63389/1‑139 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  1:430272/1‑139 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCAAGCATAGATAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAATcc                                                                                                                              >  1:719918/1‑139 (MQ=255)
   acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCACATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  1:655965/1‑139 (MQ=255)
        aGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCtaa                                                                                                                         >  2:677049/1‑139 (MQ=255)
          gATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCtaata                                                                                                                       >  2:115971/1‑139 (MQ=255)
             atCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAAtt                                                                                                                    >  2:251689/1‑139 (MQ=255)
             atCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGGCCCCTAATAAtt                                                                                                                    >  2:314464/1‑139 (MQ=255)
               cAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttg                                                                                                                  >  2:413892/1‑139 (MQ=255)
                aagaagATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 <  2:629916/139‑1 (MQ=255)
                     gaTTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            <  2:100452/139‑1 (MQ=255)
                        tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTccc                                                                                                                             <  1:408982/119‑1 (MQ=255)
                        tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTccc                                                                                                                             >  2:408982/1‑119 (MQ=255)
                               gggTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  1:677049/139‑1 (MQ=255)
                                 gTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgttgt                                                                                                <  2:396031/139‑1 (MQ=255)
                                    tGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTta                                                                                             >  2:795115/1‑139 (MQ=255)
                                          cAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAgggg                                                                                       >  2:354234/1‑139 (MQ=255)
                                              gTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                   <  1:346651/139‑1 (MQ=255)
                                              gTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                   <  1:665307/139‑1 (MQ=255)
                                                ttGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 <  1:21581/139‑1 (MQ=255)
                                                 tGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  1:137500/121‑1 (MQ=255)
                                                 tGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  >  2:137500/1‑121 (MQ=255)
                                                 tGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCaa                                                                                >  2:12271/1‑139 (MQ=255)
                                                  gCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCaaa                                                                               <  2:655965/139‑1 (MQ=255)
                                                                       tAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgg                                                          <  2:726181/139‑1 (MQ=255)
                                                                         aCCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                        <  1:413892/139‑1 (MQ=255)
                                                                          ccccATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAGAGGGGc                                                       >  2:687583/1‑139 (MQ=255)
                                                                          ccccATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGc                                                       >  1:302249/1‑139 (MQ=255)
                                                                             cATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGat                                                    >  2:414516/1‑139 (MQ=255)
                                                                               tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAAGATTCTTCGAGTAAAGGGGCGatat                                                  >  1:392782/1‑139 (MQ=255)
                                                                                       tAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtcatc                                          <  1:115971/139‑1 (MQ=255)
                                                                                         aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                    >  1:60293/1‑79 (MQ=255)
                                                                                         aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                    <  2:60293/79‑1 (MQ=255)
                                                                                         aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     >  2:772022/1‑78 (MQ=255)
                                                                                         aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     <  1:772022/78‑1 (MQ=255)
                                                                                         aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATcac                                        >  2:515163/1‑139 (MQ=255)
                                                                                          gCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATcaca                                       >  1:222709/1‑139 (MQ=255)
                                                                                           cATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtcat                                           <  2:149664/134‑1 (MQ=255)
                                                                                           cATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGtcat                                           >  1:149664/1‑134 (MQ=255)
                                                                                            aTGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     <  2:63389/139‑1 (MQ=255)
                                                                                            aTGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     <  2:430272/139‑1 (MQ=255)
                                                                                              gTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAttt                                   >  1:344436/1‑139 (MQ=255)
                                                                                              gTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAttt                                   >  2:43575/1‑139 (MQ=255)
                                                                                                aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTTTACGAGTAAAGGGGCGATATCCGtcat                                           >  2:7616/1‑129 (MQ=255)
                                                                                                aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCa                                 >  1:661734/1‑139 (MQ=255)
                                                                                                aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTACGAGTAAAGGGGCGATATCCGtcat                                           <  1:7616/129‑1 (MQ=255)
                                                                                                     atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     <  1:356874/130‑1 (MQ=255)
                                                                                                     atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     >  2:356874/1‑130 (MQ=255)
                                                                                                     atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAg                            >  2:56903/1‑139 (MQ=255)
                                                                                                        ttACGGACAGGTATCACGGGCTGTTCCATTATGAGTcccc                                                                                                                            <  1:798655/40‑1 (MQ=255)
                                                                                                        ttACGGACAGGTATCACGGGCTGTTCCATTATGAGTcccc                                                                                                                            >  2:798655/1‑40 (MQ=255)
                                                                                                         tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCAt                        >  2:653993/1‑139 (MQ=255)
                                                                                                         tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCAt                        <  1:251689/139‑1 (MQ=255)
                                                                                                            ggACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGc                     <  1:72753/139‑1 (MQ=255)
                                                                                                                aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTAt                 >  1:114035/1‑139 (MQ=255)
                                                                                                                      cACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGgag           <  2:21814/139‑1 (MQ=255)
                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAg                                                           <  1:692137/85‑1 (MQ=255)
                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAg                                                           >  2:692137/1‑85 (MQ=255)
                                                                                                                            cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGc     >  1:326823/1‑139 (MQ=255)
                                                                                                                             tGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGcc    >  2:522956/1‑139 (MQ=255)
                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGt                   >  1:122386/1‑122 (MQ=255)
                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGt                   <  2:122386/122‑1 (MQ=255)
                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAt  >  2:77614/1‑139 (MQ=255)
                                                                                                                               ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAt  >  2:244297/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577826‑4578091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: