Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 443,604 C→T S13N (AGT→AAT) 
*304* (TAG→TAA
yajL ←
panE ←
oxidative‑stress‑resistance chaperone
2‑dehydropantoate reductase, NADPH‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913443,6040CT100.0% 85.2 / NA 27S13N (AGT→AAT) 
*304* (TAG→TAA
yajL
panE
oxidative‑stress‑resistance chaperone
2‑dehydropantoate reductase, NADPH‑specific
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

TCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATA  >  NC_000913/443475‑443739
                                                                                                                                 |                                                                                                                                       
tCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                                                <  2:653598/139‑1 (MQ=255)
     cagcagCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAgg                                                                                                                           <  1:198826/139‑1 (MQ=255)
             tCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAg                                                                                                                   <  1:287781/139‑1 (MQ=255)
                     cgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                           >  1:562378/1‑139 (MQ=255)
                         aGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCt                                                                                                       <  2:585807/139‑1 (MQ=255)
                         aGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCt                                                                                                       <  2:562378/139‑1 (MQ=255)
                            aGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCat                                                                                                    <  1:183927/139‑1 (MQ=255)
                                      cAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACtctc                                                                                          >  1:157075/1‑139 (MQ=255)
                                                   cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTAcc                                                                             >  2:409493/1‑139 (MQ=255)
                                                                               tACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTa                                                 >  2:67282/1‑139 (MQ=255)
                                                                                    ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCa                                            >  1:181056/1‑139 (MQ=255)
                                                                                    ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCa                                            >  2:652178/1‑139 (MQ=255)
                                                                                                  aTCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGcgcgcg                              >  2:263643/1‑139 (MQ=255)
                                                                                                    cGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAAGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcggg                            >  1:284982/1‑139 (MQ=255)
                                                                                                      aTAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg                          <  1:457313/139‑1 (MQ=255)
                                                                                                      aTAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGCTCCGATGCGCTCATATTCACTCTCATTTCTTTTTACCATTCCAAACAGGCGTGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg                          <  2:508798/139‑1 (MQ=255)
                                                                                                            gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTa                                                 >  1:608572/1‑110 (MQ=255)
                                                                                                            gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTa                                                 <  2:608572/110‑1 (MQ=255)
                                                                                                            gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGt                    >  1:622464/1‑139 (MQ=255)
                                                                                                            gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGt                    >  1:202034/1‑139 (MQ=255)
                                                                                                            gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGt                    >  2:62047/1‑139 (MQ=255)
                                                                                                            gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGAACGGCAATCCCATGCGCGCGGGCGCGGCGt                    <  1:249293/139‑1 (MQ=255)
                                                                                                                cGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAaga                >  1:787169/1‑139 (MQ=255)
                                                                                                                  gCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAgaga              <  1:128811/139‑1 (MQ=255)
                                                                                                                   cTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaa             <  2:181056/139‑1 (MQ=255)
                                                                                                                    ttCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaaa            >  2:1883/1‑139 (MQ=255)
                                                                                                                              tCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGata  <  1:263643/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                       
TCCGCCAGCAGCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATA  >  NC_000913/443475‑443739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: