Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	450,764	C→T	A283T (GCT→ACT)	cyoA ←	cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	450,764	0	C	T	100.0%	90.0 / NA	29	A283T (GCT→ACT)	cyoA	cytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/16);  total (13/16)

CCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGC  >  NC_000913/450652‑450894                                                                                                                 |                                                                                                                                   ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaa                                                                                                          >  2:46965/1‑139 (MQ=255)       ccccTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGt                                                                                                    <  1:290073/139‑1 (MQ=255)       ccccTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGt                                                                                                    <  1:369278/139‑1 (MQ=255)          cTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTg                                                                                                 >  1:723895/1‑139 (MQ=255)                   gCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGt                                                                                        <  1:46965/139‑1 (MQ=255)                                   tCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAc                                                                        <  2:552833/139‑1 (MQ=255)                                   tCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAc                                                                        <  1:403928/139‑1 (MQ=255)                                    cATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAcc                                                                       <  1:802310/139‑1 (MQ=255)                                    cATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAcc                                                                       <  1:634048/139‑1 (MQ=255)                                            tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                    <  2:8970/134‑1 (MQ=255)                                            tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                    >  1:8970/1‑134 (MQ=255)                                                 tCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGc                                                          >  2:52741/1‑139 (MQ=255)                                                  ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGATTCATAAACTTGTTATTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCt                                                         >  1:76719/1‑139 (MQ=255)                                                  ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCt                                                         >  1:712377/1‑139 (MQ=255)                                                            gtgtGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTa                                                               <  2:316041/123‑1 (MQ=255)                                                            gtgtGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTa                                                               >  1:316041/1‑123 (MQ=255)                                                             tgtgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCa                                              <  1:52741/139‑1 (MQ=255)                                                             tgtgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCa                                              >  1:358922/1‑139 (MQ=255)                                                                         tCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACg                                  <  2:76719/139‑1 (MQ=255)                                                                            ccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               <  1:150366/139‑1 (MQ=255)                                                                            ccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               <  1:301797/139‑1 (MQ=255)                                                                              ttCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGcc                             <  2:712377/139‑1 (MQ=255)                                                                                   gctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTc                        <  1:647685/139‑1 (MQ=255)                                                                                       ggTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGAc                    >  2:776494/1‑139 (MQ=255)                                                                                                  tGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCAt                           <  2:223854/121‑1 (MQ=255)                                                                                                  tGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCAt                           >  1:223854/1‑121 (MQ=255)                                                                                                   gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGc        >  1:337348/1‑139 (MQ=255)                                                                                                        tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTg   >  2:734126/1‑139 (MQ=255)                                                                                                         aCCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc  >  1:360211/1‑139 (MQ=255)                                                                                                                 |                                                                                                                                   CCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGC  >  NC_000913/450652‑450894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑