Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 490,285 C→T *176* (TAG→TAA priC ← primosomal replication protein N''

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913490,2850CT100.0% 67.8 / NA 22*176* (TAG→TAApriCprimosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

AATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGAC  >  NC_000913/490151‑490418
                                                                                                                                      |                                                                                                                                     
aaTCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGc                                                                                                                                   >  2:385073/1‑139 (MQ=255)
          tACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAAcg                                                                                                                         >  1:606857/1‑139 (MQ=255)
          tACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAAcg                                                                                                                         >  1:603715/1‑139 (MQ=255)
                    aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTg                                                                                                               >  2:48803/1‑139 (MQ=255)
                           gcagcaCGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAg                                                                                                                                    <  1:189655/111‑1 (MQ=255)
                           gcagcaCGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAg                                                                                                                                    >  2:189655/1‑111 (MQ=255)
                                   gTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTcc                                                                                                <  2:561451/139‑1 (MQ=255)
                                       tGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAgcg                                                                                            >  2:436410/1‑139 (MQ=255)
                                              gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCa                                                                                          <  1:461025/134‑1 (MQ=255)
                                              gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCa                                                                                          >  2:461025/1‑134 (MQ=255)
                                                         cctccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCa                                                                          >  2:200069/1‑139 (MQ=255)
                                                             cagcagCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                      <  1:48803/139‑1 (MQ=255)
                                                              agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgc                                                                     <  1:200069/139‑1 (MQ=255)
                                                                   cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTc                                                                >  2:432488/1‑139 (MQ=255)
                                                                      aaTCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATa                                                             <  1:432488/139‑1 (MQ=255)
                                                                                  aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTc                                                 >  1:59328/1‑139 (MQ=255)
                                                                                                  tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCGAgcg                                                                                            >  2:658257/1‑80 (MQ=255)
                                                                                                  tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCGAgcg                                                                                            <  1:658257/80‑1 (MQ=255)
                                                                                                  tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGc                                 <  1:517051/139‑1 (MQ=255)
                                                                                                  tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGc                                 <  2:606857/139‑1 (MQ=255)
                                                                                                                               tcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAg    >  2:178716/1‑139 (MQ=255)
                                                                                                                                 cATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGAc  >  1:404333/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                     
AATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGAC  >  NC_000913/490151‑490418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: