Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	499,014	C→T	R320R (CGC→CGT)  *320* (TAG→TAA)	hemH → aes ←	ferrochelatase acetyl esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	499,014	0	C	T	100.0%	100.4 / NA	32	R320R (CGC→CGT)  *320* (TAG→TAA)	hemH aes	ferrochelatase acetyl esterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/12);  total (20/12)

CTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATG  >  NC_000913/498883‑499142                                                                                                                                    |                                                                                                                                 cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                           <  1:16218/139‑1 (MQ=255) cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                           >  2:39038/1‑139 (MQ=255) cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                           >  2:16759/1‑139 (MQ=255)          aTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTaa                                                                                                                  >  2:284145/1‑139 (MQ=255)                 aGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTg                                                                                                           >  1:407420/1‑139 (MQ=255)                     aaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAgcg                                                                                                       <  1:284145/139‑1 (MQ=255)                                     cTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCt                                                                                       >  2:152345/1‑139 (MQ=255)                                            ccGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcggc                                                                                >  2:7109/1‑139 (MQ=255)                                                  ggAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGATGAGCGGTAAAGAACTGAGCGACGTCGCGAAGAGCCTCGTTGGCGGtttt                                                                          >  2:506692/1‑139 (MQ=255)                                                     aaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                       <  1:748998/139‑1 (MQ=255)                                                                        ggCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCaaa                                                    >  2:548960/1‑139 (MQ=255)                                                                         gcgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                       >  2:725878/1‑119 (MQ=255)                                                                         gcgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                       <  1:725878/119‑1 (MQ=255)                                                                           gcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACCGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAg                                                 >  1:356836/1‑139 (MQ=255)                                                                            cTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAgg                                                >  2:484502/1‑139 (MQ=255)                                                                                   ccACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAg                                         <  1:152345/139‑1 (MQ=255)                                                                                        ccGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGc                                    <  1:7109/139‑1 (MQ=255)                                                                                                  tCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTagag                          >  2:444924/1‑139 (MQ=255)                                                                                                     aaaTGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGttt                       >  2:40717/1‑139 (MQ=255)                                                                                                           tgGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTc                 >  2:470474/1‑139 (MQ=255)                                                                                                            ggCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                >  1:86519/1‑139 (MQ=255)                                                                                                            ggCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                >  1:214830/1‑139 (MQ=255)                                                                                                                        tgCCGCGTATCGTTAAAGCTGAGCGGTAACGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg                                                                                 <  1:475170/62‑1 (MQ=255)                                                                                                                        tgCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAg                                                                                                         <  2:134507/38‑1 (MQ=255)                                                                                                                        tgCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAg                                                                                                         >  1:134507/1‑38 (MQ=255)                                                                                                                        tgCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg                                                                                 >  2:475170/1‑62 (MQ=255)                                                                                                                          ccGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATg  <  2:407420/139‑1 (MQ=255)                                                                                                                          ccGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATg  <  2:86519/139‑1 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTcgcg                                                                                               <  2:429844/43‑1 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTcgcg                                                                                               >  1:429844/1‑43 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGa                     <  1:497268/117‑1 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGa                     >  2:497268/1‑117 (MQ=255)                                                                                                                                    |                                                                                                                                 CTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATG  >  NC_000913/498883‑499142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑