Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,068,285 Δ1 bp intergenic (+31/+226) ymdF → / ← rutG KGG family protein/pyrimidine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,068,2850T.100.0% 301.7 / NA 73intergenic (+31/+226)ymdF/rutGKGG family protein/pyrimidine permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (35/38);  total (35/38)

CAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTG  >  NC_000913/1068159‑1068413
                                                                                                                              |                                                                                                                                
cagcacggcGGGAGTAATTTCAAGAATGACCCGCAGCGCGAAACTTAAGCAGGTAAACAAGGTGGTAAGAGCAGGCACGGCAAAAGCGACAACGAACCGGTGTAATCAAGGACGAATCATGGTTGCTGTAGCTCGTCaa                                                                                                                    <  1:528926/139‑1 (MQ=255)
  gcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaaaa                                                                                                                  <  1:1646755/139‑1 (MQ=255)
        cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCt                                                                                                                          >  2:1366838/1‑125 (MQ=255)
        cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCt                                                                                                                          <  1:1366838/125‑1 (MQ=255)
        cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTc                                                                                                                      >  2:1291814/1‑129 (MQ=255)
        cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTc                                                                                                                      <  1:1291814/129‑1 (MQ=255)
        cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCAt                                                                                                            >  2:1648906/1‑139 (MQ=255)
           gggTAATTTCAAGAATGACCCGCCGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAACGCCATCAc                                                                                                         >  2:528926/1‑139 (MQ=255)
           gggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAc                                                                                                         <  1:1648906/139‑1 (MQ=255)
             gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAccg                                                                                                       >  1:432856/1‑139 (MQ=255)
                 tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCgg                                                                                                   <  2:742569/139‑1 (MQ=255)
                  ttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTCGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                                                  <  2:197384/139‑1 (MQ=255)
                        aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            <  1:934061/139‑1 (MQ=255)
                            aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                                                  >  1:500015/1‑129 (MQ=255)
                            aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTAACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                                                  <  2:500015/129‑1 (MQ=255)
                             cccGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            <  1:805818/134‑1 (MQ=255)
                             cccGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            >  2:805818/1‑134 (MQ=255)
                                 cAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGAt                                                                                   >  2:336550/1‑139 (MQ=255)
                                      cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtga                                                                              >  2:1549181/1‑139 (MQ=255)
                                      cgcATCTGAAGCAGGTAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                                        >  1:787394/1‑112 (MQ=255)
                                      cgcATCTGAAGCAGGTAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                                        <  2:787394/112‑1 (MQ=255)
                                              aaGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                                                      <  2:432856/139‑1 (MQ=255)
                                                   ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            <  2:954659/112‑1 (MQ=255)
                                                   ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            >  1:954659/1‑112 (MQ=255)
                                                   ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                                                 <  1:336550/139‑1 (MQ=255)
                                                   ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                                                 >  1:1769092/1‑139 (MQ=255)
                                                     tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            >  1:1884096/1‑110 (MQ=255)
                                                     tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            <  2:1884096/110‑1 (MQ=255)
                                                        aaaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGc                                                            >  1:1227781/1‑139 (MQ=255)
                                                                gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    <  1:1954198/139‑1 (MQ=255)
                                                                     agCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                                               >  1:975004/1‑139 (MQ=255)
                                                                     agCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                                               <  2:975004/139‑1 (MQ=255)
                                                                     agCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                                               <  1:1549181/139‑1 (MQ=255)
                                                                       cAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    <  1:194888/132‑1 (MQ=255)
                                                                       cAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    >  2:194888/1‑132 (MQ=255)
                                                                               gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    >  1:323342/1‑124 (MQ=255)
                                                                               gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    <  2:323342/124‑1 (MQ=255)
                                                                                    aGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGcc                                                                                                              >  1:1789990/1‑61 (MQ=255)
                                                                                    aGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGcc                                                                                                              <  2:1789990/61‑1 (MQ=255)
                                                                                    aGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            >  2:1609790/1‑79 (MQ=255)
                                                                                    aGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            <  1:1609790/79‑1 (MQ=255)
                                                                                        aCAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa                            <  2:1227781/139‑1 (MQ=255)
                                                                                        aCAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa                            <  1:1596167/139‑1 (MQ=255)
                                                                                               accGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                                        >  2:1862917/2‑56 (MQ=255)
                                                                                               accGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                                        <  1:1862917/55‑1 (MQ=255)
                                                                                                   ggCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    <  2:1406635/104‑1 (MQ=255)
                                                                                                   ggCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    >  1:1406635/1‑104 (MQ=255)
                                                                                                       aaTCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggt                                                                                          <  1:645999/62‑1 (MQ=255)
                                                                                                       aaTCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggt                                                                                          >  2:645999/1‑62 (MQ=255)
                                                                                                       aaTCAATGACGAATCATTTTTGCTGTAGCTCGCCAAAAAGCCATCACCGCCGGTTACCCGGTGGTGGATACTGATCACCAATGAAAGCTAGCCTGATGCGTGATGCTTAACAGGCCTACTAGAAGAtccgaatagatcg             >  1:1174954/1‑127 (MQ=255)
                                                                                                        aTCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCa                                                                                                             <  2:891611/42‑1 (MQ=255)
                                                                                                        aTCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCa                                                                                                             >  1:891611/1‑42 (MQ=255)
                                                                                                            aTGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    <  2:796296/95‑1 (MQ=255)
                                                                                                            aTGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    >  1:796296/1‑95 (MQ=255)
                                                                                                             tGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    <  2:1674080/94‑1 (MQ=255)
                                                                                                             tGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                                                    >  1:1674080/1‑94 (MQ=255)
                                                                                                             tGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTg       >  2:108434/1‑139 (MQ=255)
                                                                                                               aCGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCa     <  2:1769092/139‑1 (MQ=255)
                                                                                                                 cgaatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGc                                                            <  2:1364729/78‑1 (MQ=255)
                                                                                                                 cgaatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGc                                                            >  1:1364729/6‑83 (MQ=255)
                                                                                                                  gaatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACt   <  1:108434/135‑1 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCa                                                                                                          <  1:2037625/32‑1 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCa                                                                                                          >  2:2037625/4‑35 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            >  2:1182592/4‑49 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            <  1:1182592/46‑1 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            >  1:1786370/4‑49 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                            <  2:1786370/46‑1 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                                          <  1:1251649/64‑1 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                                          >  2:1251649/4‑67 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCa                     <  1:501586/117‑1 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCa                     >  2:501586/4‑120 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTg  >  2:563125/4‑139 (MQ=255)
                                                                                                                   aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTg  >  1:1989291/4‑139 (MQ=255)
                                                                                                                              |                                                                                                                                
CAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTG  >  NC_000913/1068159‑1068413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: