Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,655,360 (C)5→4 coding (314/327 nt) ynfA ← UPF0060 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,655,3560C.100.0% 272.0 / NA 66coding (318/327 nt)ynfAUPF0060 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (37/29);  total (37/29)

AAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NC_000913/1655222‑1655490
                                                                                                                                      |                                                                                                                                      
aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTAACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                                    <  1:105397/139‑2 (MQ=255)
        aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                           <  2:1959600/139‑1 (MQ=255)
                  aaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCa                                                                                                                  >  2:1221004/1‑138 (MQ=255)
                  aaaaaaGGCGTATATTTGCACTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCa                                                                                                                  <  1:1221004/138‑1 (MQ=255)
                      aaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGAt                                                                                                                    <  2:1861291/132‑1 (MQ=255)
                      aaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCCCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGAt                                                                                                                    >  1:1861291/1‑132 (MQ=255)
                           gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                                      >  2:904325/1‑110 (MQ=255)
                           gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                                      <  1:904325/110‑1 (MQ=255)
                                         tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGc                                                                                                         >  2:830061/1‑124 (MQ=255)
                                         tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGc                                                                                                         <  1:830061/124‑1 (MQ=255)
                                         tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  2:2055618/1‑139 (MQ=255)
                                         tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  1:1823209/1‑139 (MQ=255)
                                         tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  2:1601129/1‑139 (MQ=255)
                                                 tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:1827548/139‑1 (MQ=255)
                                                   aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:604814/1‑139 (MQ=255)
                                                       aaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                                 >  1:297408/1‑83 (MQ=255)
                                                       aaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                                 <  2:297408/87‑5 (MQ=255)
                                                                   aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                                >  1:24218/1‑139 (MQ=255)
                                                                   aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTACGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       <  2:130810/116‑1 (MQ=255)
                                                                   aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTACGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       >  1:130810/1‑116 (MQ=255)
                                                                    gtACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATACAATGGATTTTTGTTCTTACGTGCGCCCCAGCGCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                               >  2:1262578/2‑139 (MQ=255)
                                                                    aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                               >  1:1065944/1‑139 (MQ=255)
                                                                     tACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGttt                                                              >  2:1928316/1‑139 (MQ=255)
                                                                      aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                             <  1:1928316/139‑1 (MQ=255)
                                                                        cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                                 >  2:829033/1‑66 (MQ=255)
                                                                        cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                                 <  1:829033/70‑5 (MQ=255)
                                                                           ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                        >  2:1794097/1‑139 (MQ=255)
                                                                            tGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  >  2:1438201/1‑112 (MQ=255)
                                                                            tGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:1438201/112‑1 (MQ=255)
                                                                             gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCg                                                                                                                              >  1:375539/1‑67 (MQ=255)
                                                                             gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCg                                                                                                                              <  2:375539/67‑1 (MQ=255)
                                                                             gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  >  2:1000815/1‑111 (MQ=255)
                                                                             gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:1000815/111‑1 (MQ=255)
                                                                                 gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaac                                                                                                                          >  1:1589350/1‑67 (MQ=255)
                                                                                 gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaac                                                                                                                          <  2:1589350/67‑1 (MQ=255)
                                                                                  cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                                    <  2:1951413/57‑2 (MQ=255)
                                                                                  cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                                    >  1:1951413/1‑56 (MQ=255)
                                                                                  cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  1:709387/1‑139 (MQ=255)
                                                                                     acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                           >  2:476481/1‑62 (MQ=255)
                                                                                     acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                           <  1:476481/62‑1 (MQ=255)
                                                                                       acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  2:1823209/139‑1 (MQ=255)
                                                                                           ccTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:1794097/139‑1 (MQ=255)
                                                                                           ccTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:2055618/139‑1 (MQ=255)
                                                                                            ctctTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:1527803/96‑1 (MQ=255)
                                                                                            ctctTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  >  2:1527803/1‑96 (MQ=255)
                                                                                                 atcttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          >  2:1349093/1‑115 (MQ=255)
                                                                                                 atcttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  1:1349093/115‑1 (MQ=255)
                                                                                                 atcttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  2:2050946/1‑139 (MQ=255)
                                                                                                   cttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                         >  2:2012753/1‑50 (MQ=255)
                                                                                                   cttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                         <  1:2012753/50‑1 (MQ=255)
                                                                                                   cttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            >  1:1933204/1‑127 (MQ=255)
                                                                                                   cttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  2:1933204/127‑1 (MQ=255)
                                                                                                     tatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              >  2:179577/1‑139 (MQ=255)
                                                                                                     tatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  1:2050946/139‑1 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                           <  1:1689080/45‑1 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                           >  2:1689080/1‑45 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              <  1:764072/122‑1 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              >  2:764072/1‑122 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:647337/128‑1 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        >  2:647337/1‑128 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  2:235708/1‑139 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:1706678/1‑139 (MQ=255)
                                                                                                      atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCACTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  2:753478/1‑139 (MQ=255)
                                                                                                            aaaaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                       >  2:1708705/1‑139 (MQ=255)
                                                                                                                 ggATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  1:433133/1‑67 (MQ=255)
                                                                                                                 ggATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          <  2:433133/67‑1 (MQ=255)
                                                                                                                    tttATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        >  2:2032951/1‑114 (MQ=255)
                                                                                                                    tttATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:2032951/114‑1 (MQ=255)
                                                                                                                            cttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          >  1:1292661/3‑88 (MQ=255)
                                                                                                                            cttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:1292661/86‑1 (MQ=255)
                                                                                                                             ttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      >  1:17523/2‑139 (MQ=255)
                                                                                                                               aCGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:1317191/1‑63 (MQ=255)
                                                                                                                               aCGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                <  2:1317191/63‑1 (MQ=255)
                                                                                                                               aCGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATaa    <  2:1706678/139‑1 (MQ=255)
                                                                                                                                 gTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGc  <  1:1708705/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
AAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NC_000913/1655222‑1655490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: