Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,821,867 (A)8→7 intergenic (‑248/+51) chbB ← / ← osmE N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,8600A.100.0% 368.8 / NA 89intergenic (‑241/+58)chbB/osmEN,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (46/43);  total (46/43)

GCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NC_000913/1821724‑1821994
                                                                                                                                        |                                                                                                                                      
gCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaa                                                                                                                                      >  2:1493828/1‑139 (MQ=255)
                          tGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGCTATGACCCTCTTCGCATTTCGGGCAAATAATTCGCGAAGATAATTAAGACCTCAGGCCAAAAAAACCGGCGTAATGGCCGGTTTCCg                                                                                                           <  1:1372351/139‑1 (MQ=255)
                            aGCAATAGTATTTCTTTGAGCTTATAAATGGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCGAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:1372351/1‑139 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:265639/1‑139 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         <  1:1493828/139‑1 (MQ=255)
                                 tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                    >  2:1251858/1‑139 (MQ=255)
                                      tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                               >  1:267955/1‑139 (MQ=255)
                                           ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTc                                                                                          >  1:392736/1‑139 (MQ=255)
                                           ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTc                                                                                          >  1:1592418/1‑139 (MQ=255)
                                             gACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                        <  2:267955/139‑1 (MQ=255)
                                               ccTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  2:392736/139‑1 (MQ=255)
                                                          gTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                             >  2:1653648/1‑121 (MQ=255)
                                                          gTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                             <  1:1653648/121‑1 (MQ=255)
                                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                                   <  2:103530/96‑1 (MQ=255)
                                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                                   >  1:103530/1‑96 (MQ=255)
                                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  2:1787639/1‑139 (MQ=255)
                                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:265639/139‑1 (MQ=255)
                                                                 cTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAATAAAACCGGCGCACTGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAg                                                                    >  1:319114/1‑139 (MQ=255)
                                                                     aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  2:1997312/139‑1 (MQ=255)
                                                                         gtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGa                                                            >  1:802513/1‑139 (MQ=255)
                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           <  2:802513/139‑1 (MQ=255)
                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGc                                                                                                                    <  2:1238441/81‑1 (MQ=255)
                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGc                                                                                                                    >  1:1238441/1‑81 (MQ=255)
                                                                           ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                >  1:1897120/1‑133 (MQ=255)
                                                                           ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  2:1897120/133‑1 (MQ=255)
                                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                                   >  2:666664/1‑110 (MQ=255)
                                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                                   <  1:666664/110‑1 (MQ=255)
                                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      <  2:1592418/139‑1 (MQ=255)
                                                                                tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             <  2:1910734/83‑1 (MQ=255)
                                                                                tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             >  1:1910734/1‑83 (MQ=255)
                                                                                    ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  1:509258/1‑139 (MQ=255)
                                                                                      tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               >  1:93743/1‑139 (MQ=255)
                                                                                      tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCACAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  1:2048283/115‑1 (MQ=255)
                                                                                      tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCACAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  2:2048283/1‑115 (MQ=255)
                                                                                       ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  2:140550/1‑139 (MQ=255)
                                                                                        ttCTCATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACGTCGCAGCCTGTGGATCAGTGTCGTATTCa                                             >  1:1325803/1‑139 (MQ=255)
                                                                                          cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGc                                           >  1:92973/1‑139 (MQ=255)
                                                                                           acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:93743/139‑1 (MQ=255)
                                                                                           acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:1325803/139‑1 (MQ=255)
                                                                                           acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:92973/139‑1 (MQ=255)
                                                                                             aTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             >  2:735392/1‑70 (MQ=255)
                                                                                             aTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             <  1:735392/70‑1 (MQ=255)
                                                                                                  gggCAAATAATTCGCGAAGGTAATTAAGAACTCAGGCCAAAAAAACCGGCCCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  2:1292198/122‑1 (MQ=255)
                                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTa                                                                                                     <  1:1788354/73‑1 (MQ=255)
                                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTa                                                                                                     >  2:1788354/1‑73 (MQ=255)
                                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    >  1:1292198/1‑122 (MQ=255)
                                                                                                   ggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAATCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  1:1451184/1‑102 (MQ=255)
                                                                                                   ggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:1451184/102‑1 (MQ=255)
                                                                                                       aataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATaa                              >  1:1529229/1‑139 (MQ=255)
                                                                                                        ataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAAc                             <  2:509258/139‑1 (MQ=255)
                                                                                                          aatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCg                           <  2:319114/139‑1 (MQ=255)
                                                                                                                  aaGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          >  2:1860727/1‑116 (MQ=255)
                                                                                                                  aaGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:1860727/116‑1 (MQ=255)
                                                                                                                     aTAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:1251858/139‑1 (MQ=255)
                                                                                                                     aTAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:2016277/139‑1 (MQ=255)
                                                                                                                         ttAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGt            <  2:1370650/139‑1 (MQ=255)
                                                                                                                             gAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:118915/105‑1 (MQ=255)
                                                                                                                             gAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          >  1:118915/1‑105 (MQ=255)
                                                                                                                              aaCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCAGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGt       <  1:1787639/139‑1 (MQ=255)
                                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:875263/74‑1 (MQ=255)
                                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  1:875263/1‑74 (MQ=255)
                                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  1:341065/74‑1 (MQ=255)
                                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  2:341065/1‑74 (MQ=255)
                                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  2:1583907/1‑139 (MQ=255)
                                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  2:1628243/1‑139 (MQ=255)
                                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  2:1935476/1‑139 (MQ=255)
                                                                                                                                cTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          >  2:1166446/1‑102 (MQ=255)
                                                                                                                                cTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:1166446/102‑1 (MQ=255)
                                                                                                                                 tCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           >  2:1124389/1‑132 (MQ=255)
                                                                                                                                 tCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  1:1124389/132‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttgt                                                                                                       >  2:268013/1‑38 (MQ=37)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttgt                                                                                                       <  1:268013/38‑1 (MQ=37)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:1910016/70‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:1959189/70‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  1:1959189/1‑70 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  1:1910016/1‑70 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:1238088/72‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     >  1:1238088/1‑72 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  2:1641893/77‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                >  1:1641893/1‑77 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCt                                                                 <  2:1819440/76‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCt                                                                 >  1:1819440/1‑76 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:89901/89‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    >  2:89901/1‑89 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          >  1:1957226/1‑99 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:1957226/99‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgat                   >  2:1097648/1‑122 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgat                   <  1:1097648/122‑1 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:664586/1‑139 (MQ=255)
                                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  1:1293205/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NC_000913/1821724‑1821994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: