Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,200,454 (A)8→7 coding (176/3633 nt) yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,200,4470A.100.0% 242.9 / NA 59coding (169/3633 nt)yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (30/29);  total (30/29)

ACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCG  >  NC_000913/2200310‑2200579
                                                                                                                                         |                                                                                                                                      
aCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAgaagaa                                                                                                                                       <  2:496508/139‑1 (MQ=255)
      aaCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaac                                                                                                                                 >  1:1733485/1‑138 (MQ=255)
                   atatAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGtt                                                                                                                   >  1:1306032/1‑139 (MQ=255)
                     ataAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTAt                                                                                                                 <  1:583304/139‑1 (MQ=255)
                        aaaaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATgag                                                                                                              <  1:1709936/139‑1 (MQ=255)
                           aaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAt                                                                                                           >  2:433654/1‑139 (MQ=255)
                              gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtttt                                                                                                        >  2:1908993/1‑139 (MQ=255)
                              gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtttt                                                                                                        >  1:1594263/1‑139 (MQ=255)
                                       cgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAg                                                                                               <  2:1943039/139‑1 (MQ=255)
                                        gcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGt                                                                                                                    >  2:787095/1‑117 (MQ=255)
                                        gcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGt                                                                                                                    <  1:787095/117‑1 (MQ=255)
                                         cTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTg                                                                                             <  1:433654/139‑1 (MQ=255)
                                                    tcgCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCa                                                                                  <  2:1519304/139‑1 (MQ=255)
                                                       cTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCa                                                                               >  2:710478/1‑139 (MQ=255)
                                                              ggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGc                                                                        >  2:1726750/1‑139 (MQ=255)
                                                               gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       <  2:1860146/139‑1 (MQ=255)
                                                                  aaCCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATc                                                                    <  2:1594263/139‑1 (MQ=255)
                                                                        tgtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                            <  1:218256/109‑1 (MQ=255)
                                                                        tgtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                            >  2:218256/1‑109 (MQ=255)
                                                                                    cTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtt                                                                                                          >  1:348164/1‑83 (MQ=255)
                                                                                    cTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtt                                                                                                          <  2:348164/83‑1 (MQ=255)
                                                                                      ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCAc                                                                                                    <  1:430585/87‑1 (MQ=255)
                                                                                      ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCAc                                                                                                    >  2:430585/1‑87 (MQ=255)
                                                                                      ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACaa                                              >  2:1551313/1‑139 (MQ=255)
                                                                                            ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTc                                        >  2:2002685/1‑139 (MQ=255)
                                                                                            ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTc                                        >  1:536014/1‑139 (MQ=255)
                                                                                               aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtt                                                                                                          <  2:1151148/72‑1 (MQ=255)
                                                                                               aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAtt                                                                                                          >  1:1151148/1‑72 (MQ=255)
                                                                                                 ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATcc                                   <  2:1733485/139‑1 (MQ=255)
                                                                                                   aGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATcccg                                 <  2:1306032/139‑1 (MQ=255)
                                                                                                         tGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                                <  1:1405136/41‑3 (MQ=255)
                                                                                                         tGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                                >  2:1405136/1‑39 (MQ=255)
                                                                                                         tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACg                                                                             <  1:227970/91‑1 (MQ=255)
                                                                                                         tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACg                                                                             >  2:227970/1‑91 (MQ=255)
                                                                                                          gggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AAcaca                                               <  1:120405/118‑1 (MQ=255)
                                                                                                          gggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AAcaca                                               >  2:120405/1‑118 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCa                                                                      <  2:963822/96‑1 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCa                                                                      >  1:963822/1‑96 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAAAAAACAAACCCTCAATcccggcccgg                           >  1:1616865/1‑139 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCt                                         >  2:504919/1‑123 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCt                                         <  1:504919/123‑1 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGAc                         >  2:1215643/1‑139 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGAc                         >  1:1189140/1‑139 (MQ=255)
                                                                                                               atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTgg                                                                                                                       <  2:1051163/43‑1 (MQ=255)
                                                                                                               atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTgg                                                                                                                       >  1:1051163/1‑43 (MQ=255)
                                                                                                               atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                            >  1:935723/1‑86 (MQ=255)
                                                                                                               atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                            <  2:935723/86‑1 (MQ=255)
                                                                                                               atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATg                     >  1:544660/1‑139 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  2:1189140/139‑1 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  1:1551313/139‑1 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  1:1908993/139‑1 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  1:710478/139‑1 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTCTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  1:1215643/139‑1 (MQ=255)
                                                                                                                         aCAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                            >  1:1958554/1‑60 (MQ=255)
                                                                                                                         aCAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                            <  2:1958554/60‑1 (MQ=255)
                                                                                                                          cAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGc          >  2:951548/1‑139 (MQ=255)
                                                                                                                            aaaaGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               >  1:84625/1‑132 (MQ=255)
                                                                                                                            aaaaGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  2:84625/132‑1 (MQ=255)
                                                                                                                                 cTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACaa                                              >  1:1849895/1‑96 (MQ=255)
                                                                                                                                 cTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACaa                                              <  2:1849895/96‑1 (MQ=255)
                                                                                                                                 cTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATc   >  1:536006/1‑139 (MQ=255)
                                                                                                                                 cTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATc   >  1:897537/1‑139 (MQ=255)
                                                                                                                                  tGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATcg  <  2:897537/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
ACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCG‑‑AACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCG  >  NC_000913/2200310‑2200579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: